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首页> 外文OA文献 >AAV vector encoding human VEGF165-transduced pectineus muscular flaps increase the formation of new tissue through induction of angiogenesis in an in vivo chamber for tissue engineering: A technique to enhance tissue and vessels in microsurgically engineered tissue
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AAV vector encoding human VEGF165-transduced pectineus muscular flaps increase the formation of new tissue through induction of angiogenesis in an in vivo chamber for tissue engineering: A technique to enhance tissue and vessels in microsurgically engineered tissue

机译：编码人VEGF165转导的果胶肌肉皮瓣的AAV载体可通过诱导体内用于组织工程的腔中的血管生成来增加新组织的形成：一种增强显微外科工程组织中组织和血管的技术

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In regenerative medicine, new approaches are required for the creation of tissue substitutes, and the interplay between different research areas, such as tissue engineering, microsurgery and gene therapy, is mandatory. In this article, we report a modification of a published model of tissue engineering, based on an arterio-venous loop enveloped in a cross-linked collagen-glycosaminoglycan template, which acts as an isolated chamber for angiogenesis and new tissue formation. In order to foster tissue formation within the chamber, which entails on the development of new vessels, we wondered whether we might combine tissue engineering with a gene therapy approach. Based on the well-described tropism of adeno-associated viral vectors for post-mitotic tissues, a muscular flap was harvested from the pectineus muscle, inserted into the chamber and transduced by either AAV vector encoding human VEGF165 or AAV vector expressing the reporter gene β-galactosidase, as a control. Histological analysis of the specimens showed that muscle transduction by AAV vector encoding human VEGF165 resulted in enhanced tissue formation, with a significant increase in the number of arterioles within the chamber in comparison with the previously published model. Pectineus muscular flap, transduced by adeno-associated viral vectors, acted as a source of the proangiogenic factor vascular endothelial growth factor, thus inducing a consistent enhancement of vessel growth into the newly formed tissue within the chamber. In conclusion, our present findings combine three different research fields such as microsurgery, tissue engineering and gene therapy, suggesting and showing the feasibility of a mixed approach for regenerative medicine.
机译：在再生医学中，需要新的方法来创建组织替代物，并且不同研究领域（例如组织工程，显微外科和基因治疗）之间的相互作用是强制性的。在本文中，我们报告了基于包裹在交联的胶原蛋白-糖胺聚糖模板中的动静脉环为基础的组织工程模型的修改，该模板充当血管生成和新组织形成的独立腔室。为了促进腔室内组织的形成，这涉及新血管的发展，我们想知道是否可以将组织工程与基因治疗方法相结合。基于对有丝分裂后组织的腺相关病毒载体的正确描述，从果胶肌肉中收获肌皮瓣，插入腔室内，然后通过编码人VEGF165的AAV载体或表达报告基因β的AAV载体进行转导-半乳糖苷酶，作为对照。样本的组织学分析表明，编码人VEGF165的AAV载体进行的肌肉转导导致组织形成增强，与先前发表的模型相比，腔室内小动脉数量显着增加。腺相关病毒载体转导的果皮肌瓣充当促血管生成因子血管内皮生长因子的来源，从而诱导持续增长的血管生长进入腔室内新形成的组织。总而言之，我们目前的发现结合了三个不同的研究领域，例如显微外科，组织工程和基因治疗，这表明并显示了混合方法用于再生医学的可行性。

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