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Engineering of cartilage tissue constructs in a 3-dimensional perfusion bioreactor culture system under controlled oxygen tension

机译：在受控氧张力下在三维灌注生物反应器培养系统中设计软骨组织构建体

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摘要

The most relevant results generated in this thesis can be summarized as follow:ud· Adult human articular chondrocytes (AHAC) from elderly individuals expanded in cultureudmedium supplemented with the growth factors TGFβ-1, FGF-2 and PDGF and subsequentlyudcultured in 3-d pellets had an enhanced chondrogenic capacity when exposed to moreudphysiological (i.e. 5%) oxygen levels.ud· In correlation with the enhanced tissue forming capacity of AHAC from elderly donors underudlow oxygen tension, the mRNA expression levels of selective matrix degrading enzymesudwere reduced as compared to conventional in vitro oxygen culture condition.ud· We developed an integrated bioreactor system, which streamlines within a single device theudphases of perfusion cell seeding and prolonged perfusion culture of cell seeded scaffolds inudvitro.ud· The culturing of uniformly seeded adult human articular chondrocytes under direct perfusion,udwhere cells are continuously exposed to a normoxic range of oxygen levels, can maintain auduniform distribution of viable cells throughout thick porous scaffolds as compared toudstatically cultured constructs.ud· The culturing of constructs uniformly seeded with adult human articular chondrocytes underuda more physiological range of oxygen resulted in a higher chondrogenic differentiation asudcompared to culture under normoxic levels. Anyhow, this effect was less pronounced asudcompared to statically cultured cell constructs or micromass cell pellets, possibly due to theudflow induced shear forces.ud· Reduced perfusion flow rates applied to chondrocytes on porous scaffolds significantlyudinduced more cartilaginous tissue in the presents of low vs. high oxygen levels. However theudeffects of low oxygen were not as marked as in pellet culture.
机译：本论文所产生的最相关结果可归纳为： ud·老年人的成人关节软骨细胞（AHAC）在培养中扩增 udmedium补充了生长因子TGFβ-1，FGF-2和PDGF，随后 udculture在3d颗粒中，当暴露于更多生理性（例如5％）的氧气水平时，其软骨形成能力增强。 ud·与氧气过低/低压力下老年供体AHAC的组织形成能力增强相关，mRNA表达水平与常规的体外氧气培养条件相比，选择性基质降解酶的含量降低了。 ud·我们开发了一个集成的生物反应器系统，该系统可以在单个设备中简化灌注细胞接种的阶段和对细胞接种支架的长时间灌注培养。 udvitro。 ud·在直接灌注下培养均匀播种的成年人类关节软骨细胞，其中细胞连续暴露于常氧范围氧水平可以使活细胞在整个多孔多孔支架中保持均匀分布，而不是经过人工培养的成体细胞。ud在人的生理范围更大的氧气范围内培养均匀接种成年人软骨细胞的结果是在常氧水平下，软骨形成的分化与培养相比。无论如何，这种作用不如静态培养的细胞构建体或微团细胞沉淀明显，可能是由于 udflow诱导的剪切力。 ud·多孔支架上软骨细胞的灌注流速降低显着 ud诱导了软骨组织的更多软骨形成低氧水平与高氧水平的关系。但是，低氧的影响不如沉淀培养中的明显。

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    Ströbel Simon;

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  • 年度 2007
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  • 正文语种 {"code":"en","name":"English","id":9}
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