• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Development of supported biomimetic membranes for insertion of aquaporin protein water channels for novel water filtration applications
【2h】

Development of supported biomimetic membranes for insertion of aquaporin protein water channels for novel water filtration applications

机译：开发支持的仿生膜，用于插入水通道蛋白水通道，用于新型水过滤应用

页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aquaporins represent a class of membrane protein channels found in all living organisms that selectively transport water molecules across biological membranes. The work presented in this thesis was motivated by the conceptual idea of incorporating aquaporin water channels into biomimetic membranes to develop novel water separation technologies. To accomplish this, it is necessary to construct an efficient platform to handle biomimetic membranes. Moreover, general methods are required to reliable and controllable reconstitute membrane proteins into artificially made model membranes. These are the topics of this thesis, and are divided into three main chapters. Chapter 2 reviews recent advances in the design and construction of biomimetic membrane arrays. Moreover, current and novel strategies for the reconstitution of membrane proteins into biomimetic membranes are reviewed. Chapter 3 presents the development of biomimetic membrane devices suitable of supporting the establishment of functional biomimetic membrane arrays. Furthermore, scaling up the effective membrane area from rectangular 8×8 arrays (64 membranes) to rectangular 24×24 (576 membranes) or hexagonal 24×27 (648 membranes) is demonstrated in a horizontal chamber design. Chapter 4 characterizes reconstitution and folding of E. coli Aquaporin–Z (AqpZ) and the spinach plasma integral protein 2;1 (SoPIP2;1) aquaporins into model membranes. A central part of this chapter is the development of a method for formation of giant protein vesicles (≥10 μm). This constitutes a new methodology to correctly and functionally reconstitute membrane proteins in controllable amounts into giant vesicles. The method for formation of giant protein vesicles subsequently led to the first functional prototype of an aquaporin-membrane water filtration device.
机译：水通道蛋白代表在所有活生物体中发现的一类膜蛋白通道，可选择性地将水分子跨过生物膜运输。本文提出的工作是受将水通道蛋白水通道结合到仿生膜中以开发新型水分离技术的概念启发的。为此，必须构建一个有效的平台来处理仿生膜。此外，需要通用方法将可靠且可控的重组膜蛋白重构为人工制作的模型膜。这些是本文的主题，分为三个主要章节。第2章回顾了仿生膜阵列设计和构造的最新进展。此外，综述了将膜蛋白重构为仿生膜的当前和新颖策略。第3章介绍了仿生膜设备的发展，该设备适用于支持功能仿生膜阵列的建立。此外，在水平腔室设计中展示了将有效膜面积从矩形8×8阵列（64个膜）扩大到矩形24×24（576个膜）或六角形24×27（648个膜）。第4章描述了大肠杆菌水通道蛋白Z（AqpZ）和菠菜血浆整合蛋白2; 1（SoPIP2; 1）水通道蛋白在模型膜中的重构和折叠。本章的中心是开发一种形成巨大蛋白囊泡（≥10μm）的方法。这构成了一种新方法，可以将可控数量的膜蛋白正确和功能性地重组为巨大的囊泡。形成巨大蛋白囊泡的方法随后导致了水通道蛋白膜水过滤装置的第一个功能原型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号