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A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device.

机译：一种新颖的温度控制方法，用于缩短热循环时间，以在一次性聚合物微流体装置中实现快速聚合酶链式反应（pCR）。

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摘要

We present a new temperature control method capable of effectively shortening the thermal cycling time of polymerase chain reaction (PCR) in a disposable polymer microfluidic device with external heater and temperature sensor. The method employs optimized temperature overshooting and undershooting steps to achieve a rapid ramping between the temperature steps for DNA denaturation, annealing and extension. The temperature dynamics within the microfluidic PCR chamber was characterized and the overshooting and undershooting parameters were optimized using the temperature dependent fluorescence signal from Rhodamine B. The method was validated with PCR amplification of mecA gene (162 bp) from Methicillin-resistant Staphylococcus aureus bacterium (MRSA), where the time for 30 cycles was reduced from 50 min (without over- and undershooting) to 20 min.

机译：我们提出了一种新的温度控制方法，该方法能够有效地缩短带有外部加热器和温度传感器的一次性聚合物微流体装置中聚合酶链反应（PCR）的热循环时间。该方法采用优化的温度超调和下调步骤，以实现DNA变性，退火和延伸的温度步骤之间的快速上升。表征了微流体PCR室中的温度动态，并使用了若丹明B产生的温度依赖性荧光信号来优化过冲和下冲参数。通过PCR扩增耐甲氧西林金黄色葡萄球菌细菌（mecA基因）（162 bp）验证了该方法的有效性。 MRSA），其中30个周期的时间从50分钟（没有过冲和下冲）减少到20分钟。

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Bu Minqiang; R. Perch-Nielsen Ivan; Sørensen Karen Skotte; Skov Julia; Yi Sun; Bang Dang Duong; E. Pedersen Michael; Hansen Mikkel Fougt; Wolff Anders;
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年度 2012
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正文语种 eng
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1. A temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device [J] . Minqiang Bu, Ivan R. Perch-Nielsen, Karen S. Sorensen, Journal of Micromechanics and Microengineering . 2013,第7期

机译：一种用于缩短热循环时间以在一次性聚合物微流体装置中实现快速聚合酶链反应（PCR）的温度控制方法
2. Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification [J] . Shadi Mahjoob, Kambiz Vafai, N. Reginald Beer International Journal of Heat and Mass Transfer . 2008,第9a10期

机译：快速微流体热循环仪，用于聚合酶链反应核酸扩增
3. Evaluation of Polymerase Chain Reaction (PCR)-Based Methods for Rapid, Accurate Detection and Monitoring of Verticillium dahliae in Woody Hosts by Real-Time PCR (vol 99, pg 866, 2015) [J] . Aljawasim B., Vincelli P. Plant Disease . 2015,第10期

机译：基于聚合酶链反应（PCR）的实时PCR快速，准确检测和监测木本寄主中黄萎病的方法的评估（第99卷，第866页，2015年）
4. Thermal Cycling Control System Design for Polymerase Chain Reaction(PCR) DNA Amplification [C] . Dochang Oh, Yangwon Lee Society of Instrument and Control Engineers . 2005

机译：聚合酶链反应的热循环控制系统设计（PCR）DNA扩增
5. Immobilizations for an aluminum nitride (AlN) based biosensor and real-time polymerase chain reaction (RT-PCR) device. [D] . Hughes, Chantelle. 2007

机译：基于氮化铝（AlN）的生物传感器和实时聚合酶链反应（RT-PCR）装置的固定化。
6. Real‐time identification of Pseudomonas aeruginosa direct from clinical samples using a rapid extraction method and polymerase chain reaction (PCR) [O] . Richard I. Jaffe, Janae D. Lane, Christopher W. Bates 2001

机译：使用快速提取方法和聚合酶链反应（PCR）直接从临床样品中实时鉴定铜绿假单胞菌
7. Evaluation of Polymerase Chain Reaction (PCR)-Based Methods for Rapid, Accurate Detection and Monitoring of Verticillium dahliae in Woody Hosts by Real-Time PCR [O] . Baker Aljawasim, Paul Vincelli 2015

机译：通过实时PCR评估聚合酶链反应（PCR）的基于木质宿主中的快速，精确地检测和监测伐木菌的快速，精确检测和监测的方法

A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device.

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