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首页> 外文OA文献 >Increasing levels of dietary crystalline methionine affect plasma methionine profiles, ammonia excretion, and the expression of genes related to the hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)
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Increasing levels of dietary crystalline methionine affect plasma methionine profiles, ammonia excretion, and the expression of genes related to the hepatic intermediary metabolism in rainbow trout (Oncorhynchus mykiss)

机译：日益增加的膳食结晶蛋氨酸水平影响虹鳟（Oncorhynchus mykiss）的血浆蛋氨酸谱，氨排泄和与肝脏中间代谢相关的基因表达

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摘要

Strictly carnivorous fish with high requirements for dietary protein, such as rainbow trout (Oncorhynchus mykiss) are interesting models for studying the role of amino acids as key regulators of intermediary metabolism. Methionine is an essential amino acid for rainbow trout, and works as a signalling factor in different metabolic pathways. The study investigated the effect of increasing dietary methionine intake on the intermediary metabolism in the liver of juvenile rainbow trout. For this purpose, five diets were formulated with increasing methionine levels from 0.60 to 1.29% dry matter. The diets were fed in excess for six weeks before three sampling campaigns carried out successively to elucidate (i) the hepatic expression of selected genes involved in lipid, glucose and amino acid metabolism; (ii) the postprandial ammonia excretion; and (iii) the postprandial plasma methionine concentrations. The transcript levels of enzymes involved in lipid metabolism (fatty acid synthase, glucose 6 phosphate dehydrogenase and carnitine palmitoyl transferase 1 a), gluconeogenesis (fructose-1,6-biphosphatase) and amino acid catabolism (alanine amino transferase and glutamate dehydrogenase) were significantly affected by the increase in dietary methionine. Changes in gene expression reflected to some extent the decrease in ammonia excretion (P=0.022) and in the hepatosomatic index (HSI; P
机译：对膳食蛋白质有高要求的严格食肉鱼类，例如虹鳟（Oncorhynchus mykiss），是研究氨基酸作为中间代谢关键调节剂的作用的有趣模型。蛋氨酸是虹鳟鱼的必需氨基酸，并在不同的代谢途径中起信号传导因子的作用。该研究调查了饮食中蛋氨酸摄入量的增加对幼年虹鳟鱼肝脏中间代谢的影响。为此，配制了五种日粮，使蛋氨酸的干物质含量从0.60增加到1.29％。在连续进行三个采样运动以阐明之前，这些饮食被过量喂养了六个星期，以阐明：（i）涉及脂质，葡萄糖和氨基酸代谢的选定基因在肝脏中的表达； （ii）餐后氨气排泄； （iii）餐后血浆蛋氨酸浓度。参与脂类代谢的酶（脂肪酸合酶，葡萄糖6磷酸脱氢酶和肉碱棕榈酰转移酶1a），糖异生（果糖-1,6-双磷酸酶）和氨基酸分解代谢（丙氨酸氨基转移酶和谷氨酸脱氢酶）的转录水平显着提高。受到饮食中蛋氨酸含量增加的影响。基因表达的变化在一定程度上反映了氨排泄的减少（P = 0.022）和肝体指数的降低（HSI； P

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