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High density culture of marine microalgae using semi-continuous and continuous systems.

机译：使用高密度培养的海洋微藻的半连续和连续系统。

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摘要

This study entitled "High density culture o f marine micro-algae using semicontinuous and continuous systems" was carried out with the objectives of devising small-scale low costudsystems for use in laboratory conditions. The study was carried out using two commonly used species of microalgae viz., the flagellate Isochrysis galbana and the diatom, Chaetoceros sp.ud The system devised was a modified turbidostat (2 1 to 5 1) having three tier gravitational flow with manual controllers and without automation. For scale-up semicontinuous culture, a 60 1 capacity internally illuminated plexy glass culture system was devised.ud Using 2.5 times the normal nutrient concentration, limited supply o f CO2 , activated charcoal filtered air and under sterile conditions it was possible to obtain upto 5 fold increase in culture cell density when compared to the normal batch cultures. Besides it was possible to maintain the cultures for durations varying from 2 to 5 weeks without collapse. In the continuous culture system o f 2 I capacity . The daily harvest o f high density culture amounted to 500 ml,udand in the semi-continuous system (4 1 capacity). The once-in-3 day harvest varied between 2-3 litres. From a single 60 1 capacity semicontinuous system running for 36 days the totaludlarvest amounted to 530 litres o f average 3.2 million cells/ml culture.ud With respect to maricuhure, the application of conclusions derived from present.work would resut in increased efficiency and reduced cost of production of microalgae.
机译：这项名为“使用半连续和连续系统的海洋微藻高密度培养”的研究旨在设计用于实验室条件的小型低成本 uds系统。该研究是使用两种常见的微藻物种，即鞭毛等鞭藻和硅藻Chaetoceros sp.ud进行的。设计的系统是带有手动控制器的三层重力流的改进型涡轮变阻器（2 1至5 1）。并且没有自动化。为了扩大规模的半连续培养，设计了60 1容量的内部照明有机玻璃培养系统。 ud使用正常营养浓度的2.5倍，有限的CO2供给，活性炭过滤的空气以及在无菌条件下最多可获得5种与正常分批培养相比，培养细胞密度增加了两倍。此外，可以将培养物维持2到5周的持续时间而不会崩溃。在连续培养系统中，容量为2 I。在半连续系统（4 1容量）中，高密度培养物的每日收获量为500 ml。 3天一次的收获量介于2-3升之间。从一个连续运行60天的60 1容量的半连续系统中，总提取量为530升，平均320万个细胞/ ml培养物。 ud对于海水消毒，应用从现阶段得出的结论将提高效率。并降低了微藻的生产成本。

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    Bharguram Lambade Sunil;

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  • 年度 1999
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