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Botrytone, a new naphthalenone pentaketide produced by botrytis fabae, the causal agent of chocolate spot disease on vicia faba

机译:Botrytone,一种由蚕豆(botrytis fabae)产生的新型萘酮五肽,是蚕豆上巧克力斑病的致病因子

摘要

A strain of Botrytis fabae isolated from faba bean (Vicia faba L.) plants displaying clear chocolate spot disease symptoms produced phytotoxic metabolites in vitro. The phytotoxins isolated from the culture filtrate organic extract were characterized by spectroscopic and optical methods. A new naphthalenone pentaketide, named botrytone, was isolated and characterized as (4R)-3,4-dihydro-4,5,8-trihydroxy-1(2H)-naphthalenone together with other well-known closely related naphthalenones such as regiolone and cis- and trans-3,4-dihydro-2,4,8-trihydroxynaphthalen-1(2H)-ones. When tested on leaves of the host plant, with the cis- and trans-3,4-dihydro-2,4,8- trihydroxynaphthalen-1(2H)-ones assayed in mixture, regiolone demonstrated the highest level of phytotoxicity together with cis- and trans-3,4-dihydro-2,4,8- trihydroxynaphthalen-1(2H)-ones. Botrytone showed moderate phytotoxic activity at 1 mg/mL and was still phytotoxic at 0.5 mg/mL.
机译:从蚕豆(Vicia faba L.)植物中分离出的Botrytis fabae菌株显示出明显的巧克力斑点病症状,在体外产生了植物毒性代谢产物。从培养滤液有机提取物中分离出的植物毒素通过光谱和光学方法进行表征。分离出一种新的萘酮五肽,称为Botrytone,并将其表征为(4R)-3,4-dihydro-4,5,8-trihydroxy-1(2H)-萘酮以及其他众所周知的密切相关的萘酮,如雷格隆和顺式和反式3,4-二氢-2,4,8-三羟基萘-1(2H)-ones。当在宿主植物的叶子上进行测试时,在混合物中测定了顺式和反式-3,4-二氢-2,4,8-三羟基萘-1(2H)-的含量,瑞吉隆与顺式一起显示出最高的植物毒性。 -和反式-3,4-二氢-2,4,8-三羟基萘-1(2H)-。葡萄孢菌素在1 mg / mL时显示中度植物毒性活性,而在0.5 mg / mL时仍具有植物毒性。

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