Provided by present application are a reference signal transmission method and device, which relate to the technical field of communication and are used to realize frequency hopping transmission of a reference signal when a PRB-interleaved resource allocation mode is employed. The method comprises: a terminal device receives first configuration information from an access network device, the first configuration information being used to instruct the terminal device to transmit frequency domain resources of a reference signal at at least two moments; the terminal device determines a physical resource block (PRB) cluster corresponding to each of the at least two moments according to the first configuration information, wherein one PRB cluster is composed of multiple PRBs that are equally spaced apart and are discontinuous; and the terminal device sends the reference signal to the access network device on the PRB cluster corresponding to each moment.
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