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OPTIMIZED ECONOMIC / VECTOR SYSTEM FOR GENERATING PROTECTIVE MONO AND MULTIVALENTER

机译:用于生成保护性单价和多价确认单的优化经济/矢量系统

摘要

The invention relates to recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of the highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for generating a protective immune response against pathogens. The invention provides in particular K. lactis strains for the targeted cloning of foreign antigen-coding nucleic acids into the yeast genome of the K. lactis strain, which is characterized in that the K. lactis strain has integrated expression cassettes for foreign antigens as an alternative or in addition to the KILAC4 locus on the KIURA3-20 locus (KLLA0E22771g) and/or on the KIMET5-1 locus (KLLA0B03938g). The invention further relates to integrative expression vectors and to methods for producing the K. lactis strains of the invention as well as to the use thereof as vaccines.
机译:本发明涉及重组克鲁维酵母(Kluyveromyces lactis)酵母,该酵母能够高效表达一种或多种外源蛋白质,并且适合用作疫苗以产生针对病原体的保护性免疫应答。本发明特别提供了乳酸克鲁维酵母菌株,用于将外源抗原编码核酸靶向克隆到乳酸克鲁维酵母菌株的酵母基因组中,其特征在于,乳酸克鲁维酵母菌株具有整合的外源抗原表达盒,作为外源抗原。在KIURA3-20基因座(KLLA0E22771g)和/或KIMET5-1基因座(KLLA0B03938g)上替代或除KILAC4基因座。本发明进一步涉及整合表达载体和生产本发明乳酸克鲁维酵母菌株的方法,以及其作为疫苗的用途。

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