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DIAGNOSTIC TECHNIQUE FOR SEVERE PREECLAMPSIA IN PREGNANT WOMEN

机译:妊娠妇女重度青光眼的诊断技术

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to obstetrics, gynaecology, molecular biology, molecular genetics, biochemistry, and can be used for diagnosing severe preeclampsia in pregnant women. Blood plasma of the pregnant woman is examined for concentration of extracellular DNA (ec DNA), number of copies of ribosomal DNA in extracellular DNA (ec rRNA), number of copies of mitochondrial DNA in extracellular DNA (ec mtDNA), nuclease activity of DNase 1. Nuclear leukocyte DNA is used to determine the number of copies of genomic ribosomal DNA (rDNA genome), number of copies of genomic mitochondrial DNA (mtDNA genome). If the ec DNA is more than 1000 ng/ml, ec rDNA more than 1000, ec mtDNA more than 200, the ratio of ec rDNA to the rDNA genome is more than 2, the ratio of ec mdDNA to the mtDNA genome is more than 1.64, the nuclease activity of DNase 1 is more than 10 units/ml, is diagnosed severe preeclampsia.;EFFECT: method provides accurate detection of severe preeclampsia by determining a set of molecular-biological factors - criteria for objective reliable diagnosis of severe asymptomatic forms of preeclampsia.;1 cl, 3 ex
机译:技术领域本发明涉及医学,即涉及妇产科,妇科,分子生物学,分子遗传学,生化的医学,可用于诊断孕妇的严重先兆子痫。检查孕妇血浆中的细胞外DNA(ec DNA)浓度,细胞外DNA(ec rRNA)中核糖体DNA的拷贝数,细胞外DNA(ec mtDNA)中线粒体DNA的拷贝数,DNase的核酸酶活性1.核白细胞DNA用于确定基因组核糖体DNA(rDNA基因组)的拷贝数,基因组线粒体DNA(mtDNA基因组)的拷贝数。如果ec DNA大于1000 ng / ml,ec rDNA大于1000,ec mtDNA大于200,则ec rDNA与rDNA基因组的比率大于2,ec mdDNA与mtDNA基因组的比率大于2。 1.64,DNase 1的核酸酶活性超过10单位/ ml,被诊断为严重先兆子痫。效果:该方法可通过确定一组分子生物学因素来准确检测严重先兆子痫-客观可靠地诊断严重无症状形式的标准子痫前期。; 1 cl,3 ex

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