首页> 外国专利> GENE-THERAPEUTIC DNA-VECTOR BASED ON GENE-THERAPEUTIC DNA-VECTOR VTVAF17, CARRYING TARGET GENE SELECTED FROM GROUP OF GENES COL1A1, COL1A2, P4HA1, P4HA2, COL7A1, CLCA2, ELN, PLOD1 TO INCREASE LEVEL OF EXPRESSION OF SAID TARGET GENES, METHOD FOR PRODUCTION AND USE THEREOF, STRAIN ESCHERICHIA COLI SCS110-AF/VTVAF17-COL1A1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-COL1A2, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-P4HA1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-P4HA2, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-COL7A1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-CLCA2, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-ELN, OR ESCHERICHIA COLI SCS110-AF/VTVAF17- PLOD1, CARRYING GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, METHOD FOR INDUSTRIAL PRODUCTION OF GENE-THERAPEUTIC DNA VECTOR

GENE-THERAPEUTIC DNA-VECTOR BASED ON GENE-THERAPEUTIC DNA-VECTOR VTVAF17, CARRYING TARGET GENE SELECTED FROM GROUP OF GENES COL1A1, COL1A2, P4HA1, P4HA2, COL7A1, CLCA2, ELN, PLOD1 TO INCREASE LEVEL OF EXPRESSION OF SAID TARGET GENES, METHOD FOR PRODUCTION AND USE THEREOF, STRAIN ESCHERICHIA COLI SCS110-AF/VTVAF17-COL1A1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-COL1A2, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-P4HA1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-P4HA2, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-COL7A1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-CLCA2, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-ELN, OR ESCHERICHIA COLI SCS110-AF/VTVAF17- PLOD1, CARRYING GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, METHOD FOR INDUSTRIAL PRODUCTION OF GENE-THERAPEUTIC DNA VECTOR

机译：基于基因-治疗性DNA-载体VTVAF17的基因-治疗性DNA-载体，携带选自基因组COL1A1，COL1A2，P4HA1，P4HA2，COL7A1，CLCA2，ELN，PLOD1的靶标基因，可提高基因表达水平为了生产和使用，应使用埃希氏菌SCS110-AF / VTVAF17-COL1A1或埃希氏菌SCS110-AF / VTVAF17-COL1A2或埃希氏菌SCS110-AF / VTVAF17-P4HA1或埃希氏菌SCS110-AF4或ESCHERICHIA COLI SCS110-AF / VTVAF17-COL7A1或ESCHERICHIA COLI SCS110-AF / VTVAF17-CLCA2或ESCHERICHIA COLI SCS110-AF / VTVAF17-ELN或ESCHERICHIA COLI SCS110-AF / VTVAFPE17-PLOD1，携带基因，基因治疗方法，基因治疗性DNA载体的工业生产方法

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FIELD: biotechnology; medicine; agriculture.;SUBSTANCE: invention refers to genetic engineering and can be used in biotechnology, medicine and agriculture to develop gene therapy preparations. Presented is a gene-therapeutic DNA vector based on the gene-therapeutic DNA vector VTvaf17, carrying a target gene selected from a group of COL1A1, COL1A2, P4HA1, P4HA2, COL7A1, CLCA2, ELN, PLOD1 genes to increase the level of expression of this target gene in the human body and animals. Gene-therapeutic DNA vector VTvaf17-COL1A1, or VTvaf17-COL1A2, or VTvaf17-P4HA1, or VTvaf17-P4HA2, or VTvaf17-COL7A1, or VTvaf17-CLCA2, or VTvaf17-ELN, or VTvaf17-PLOD1 has nucleotide sequence SEQ ID No.1, or SEQ ID No. 2, or SEQ ID No. 3, or SEQ ID No. 4, or SEQ ID No. 5, or SEQ ID No. 6, or SEQ ID No. 7, or SEQ ID No. 8, respectively. Invention also discloses a method of producing said vector, use of a vector, an Escherichia coli strain carrying said vector, as well as a method of producing said vector on an industrial scale.;EFFECT: invention provides the possibility of its safe application for genetic therapy of humans and animals.;22 cl, 23 dwg, 32 ex

机译：领域：生物技术;药物;物质：发明是指基因工程，可以用于生物技术，医学和农业中以开发基因疗法的制剂。提出了一种基于基因治疗DNA载体VTvaf17的基因治疗DNA载体，该载体携带选自COL1A1，COL1A2，P4HA1，P4HA2，COL7A1，CLCA2，ELN和PLOD1基因的靶基因，以增加其表达水平这个靶基因在人体和动物中。基因治疗性DNA载体VTvaf17-COL1A1或VTvaf17-COL1A2或VTvaf17-P4HA1或VTvaf17-P4HA2或VTvaf17-COL7A1或VTvaf17-CLCA2或VTvaf17-ELN或VTvaf17-ODN序列具有SEQvaf17 1，或SEQ ID No.2，或SEQ ID No.3，或SEQ ID No.4，或SEQ ID No.5，或SEQ ID No.6，或SEQ ID No.7，或SEQ ID No.8 ，分别。本发明还公开了产生所述载体的方法，载体的用途，带有所述载体的大肠杆菌菌株以及工业规模生产所述载体的方法。效果：本发明提供了安全用于人类和动物基因治疗的可能性。; 22 cl，23 dwg，32 ex

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公开/公告号RU2730661C2

专利类型
公开/公告日2020-08-24

原文格式PDF
申请/专利权人 CELL AND GENE THERAPY LTD;
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申请/专利号RU20180147082
发明设计人 SAVELIEVA NATALIA (AT);
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申请日2018-12-27
分类号C12N15/12;C12N15/70;C12N1/21;A61K48;C12R1/21;
国家 RU
入库时间 2022-08-21 11:02:20

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