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METHOD OF PRODUCING GISELA 5 AND AFL-2 ROOTSTOCK IN IN VITRO

机译:体外制备Gisela 5和AFL-2根的方法

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology. Invention is a method for producing Gisela rootstock 5 and AFL-2 in vitro, including propagation of in-vitro fertilizer plants and single-stem grafts cutting on agarized nutrient medium containing macro- and microelements and vitamins based on nutrient medium Murashige and Skoog, where produced sprouts are transplanted into Murashige and Skoog culture medium with higher concentration of salts - chelate Fe 50 mg/l and Ca(NO3)2 1000 mg/l, at that shoots of large size - 5–6 cm - are used for reproduction, and sprouts of smaller size transplanted on nutrient medium with content of 0.1 mg 6 Bap., wherein the transplantation is to be performed in 3–4 weeks, wherein vitreous cultures are rejected, and culturing is carried out under optimal conditions: temperature - 22–26 degrees, illumination - 2000–5000 lux, with 16-hour photoperiod.;EFFECT: invention increases output of healthier plants.;1 cl
机译:技术领域本发明涉及生物技术领域。本发明是一种体外生产Gisela砧木5和AFL-2的方法,包括体外肥料植物的繁殖和在营养培养基Murashige和Skoog的基础上,在含有大量和微量元素和维生素的琼脂化营养培养基上切割单茎移植物,其中将产生的新芽移植到具有较高盐浓度的Murashige和Skoog培养基中-螯合Fe 50 mg / l和Ca(NO 3 2 1000 mg / l使用大尺寸的芽(5–6厘米)进行繁殖,将小尺寸的芽苗移植到营养培养基中,芽苗含量为0.1 mg 6 Bap。,移植时间为3-4周,其中需进行玻璃体培养拒绝,并在最佳条件下进行培养:温度-22-26度,光照-2000-5000 lux,光照时间为16小时;效果:发明增加了更健康植物的产量。1cl

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