首页> 外国专利> METHOD FOR IDENTIFICATION OF DNA OF DOMESTIC DOG TISSUE (CANIS LUPUS FAMILIARIS) IN DRY FODDER AND MEAT SEMI-PRODUCTS

METHOD FOR IDENTIFICATION OF DNA OF DOMESTIC DOG TISSUE (CANIS LUPUS FAMILIARIS) IN DRY FODDER AND MEAT SEMI-PRODUCTS

机译：干饲料和肉类半成品中家犬组织DNA的鉴定方法

页面导航

摘要
著录项
相似文献

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology. Invention is a method for identification of DNA of domestic dog tissue (Canis lupus familiaris) in dry fodder and meat semi-products, involving DNA recovery from animal tissue by sorption method, polymerase chain reaction with fluorescent detection is carried out with 45 cycles of real-time amplification using oligonucleotide primers, probes, fluorescent dyes specific for the DNA genome region: for a specific signal for an animal and Cy5/Red for an internal control sample in form of a suspension of bacteriophage T4 with concentration of 5 × 10³ of phage particles per 1 mcl, positive control sample in the form of a mixture containing fragments of animal genomes and bacteriophage T4 with a nucleotide sequence: T4F TACATATAAATCACGCAAAGCT4R TAGTATGGCTAATCTTATTGG T4P CY5 ACATTGGCACTGACCGAGTTC, taken in ratio 1:1, and measurement of accumulation of fluorescent signals via channels of corresponding fluorescent dyes, interpretation of results based on the presence or absence of the intersection of the fluorescence curve with the threshold line, if the fluorescent signal accumulation curves extend to 35 cycles, then the result of the reaction is considered to be positive, and if the curves do not cross the threshold line or cross it after 35 cycles, the result of the reaction is negative, according to the invention, DNA is recovered from domestic dog tissue (Canis lupus familiaris) and the internal control sample is T4 bacterial phagolysate, and for the positive control sample - native T4 bacteriophage genome fragments and domestic dog tissue (Canis lupus familiaris) with the following nucleotide sequence: Canis F ATTCggCCTACATCCgTgAC forward primer Canis R AgAAgACCCCTgCTACgACT reverse primer Canis P FAM-CTTgAgTggAgTAgggCgg-BHQ1 probe, wherein for DNA of dog tissue fluorescent dye FAM/Green is used.;EFFECT: invention makes it possible to increase accuracy of species identity identification and to simplify the sampling process.;1 cl, 5 tbl

机译：技术领域本发明涉及生物技术领域。本发明是一种在干饲料和肉半成品中鉴定家犬组织DNA的方法，涉及通过吸附法从动物组织中回收DNA，用荧光循环的聚合酶链式反应进行荧光检测，实际循环45次。使用寡核苷酸引物，探针，DNA基因组区域特有的荧光染料进行实时扩增：针对动物的特定信号以及针对内部对照样品的Cy5 / Red，以浓度为5×10 3 阳性对照样品，其混合物形式为含有动物基因组片段和噬菌体T4的核苷酸序列：T4F TACATATAAATCACGCAAAGCT4R TAGTATGGCTAATCTTATTGG T4P CY5 ACATTGGCACTGACCGAGTTC，比例为1：1，以及通过相应的荧光染料通道测量荧光信号的积累，根据是否存在荧光来解释结果荧光曲线与阈值线的交点，如果荧光信号累积曲线延伸到35个循环，则反应结果被认为是阳性的，并且如果曲线未越过阈值线或在35个循环后未穿过阈值线，根据本发明，该反应的结果是阴性的，从家犬组织中回收DNA，而内部对照样品是T4细菌吞噬物，而阳性对照样品是天然T4噬菌体基因组片段和家犬组织（Canis lupus Friendlyis）具有以下核苷酸序列：Canis F ATTCggCCTACATCCgTgAC正向引物Canis R AgAAgACCCCTgCTACgACT反向引物Canis P FAM-CTTgAgTggAgTAgggCgg-BHQ1探针，其中对于犬组织的DNA使用了荧光染料FAM / Green。可以提高物种身份识别的准确性并简化采样过程。1cl，5 tbl

著录项

公开/公告号RU2728612C1

专利类型
公开/公告日2020-07-30

原文格式PDF
申请/专利权人 FEDERALNOE GOSUDARSTVENNOE BYUDZHETNOE OBRAZOVATELNOE UCHREZHDENIE VYSSHEGO OBRAZOVANIYA KUBANSKIJ GOSUDARSTVENNYJ AGRARNYJ UNIVERSITET IMENI I.T. TRUBILINA;
展开▼

申请/专利号RU20190133140
发明设计人 CHERNYKH OLEG YUREVICH (RU);BANNOV VASILIJ ALEKSANDROVICH (RU);MALYSHEV DENIS VLADISLAVOVICH (RU);CHERNYKH VLADIMIR OLEGOVICH (RU);LYSENKO ALEKSANDR ANATOLIEVICH (RU);KOTELNIKOVA ALEKSANDRA ANDREEVNA (RU);KOSHCHAEV ANDREJ GEORGIEVICH (RU);KRIVONOS ROMAN ANATOLEVICH (RU);DROBIN YURIJ DMITRIEVICH (RU);SHEVKOPLYAS VLADIMIR NIKOLAEVICH (RU);SHEVCHENKO ALEKSANDR ALEKSEEVICH (RU);KHAKHOV LATIF ASLANBIEVICH (RU);BYKOVA OLGA ALEKSANDROVNA (RU);LORETTS OLGA GENNADEVNA (RU);KRIVONOGOVA ANNA SERGEEVNA (RU);KOLOMIETS SERGEJ NIKOLAEVICH (RU);ZABASHTA NIKOLAJ NIKOLAEVICH (RU);
展开▼

申请日2019-10-16
分类号C12Q1/68;
国家 RU
入库时间 2022-08-21 11:02:22

相似文献

专利
外文文献
中文文献