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METHOD FOR INTRAOPERATIVE ASSESSMENT OF INTESTINAL WALL VIABILITY IN AN EXPERIMENTAL MODEL ON RABBITS

机译:兔子实验模型中肠壁可行性的术中评估方法

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to surgery of abdominal organs, and can be used for intraoperative assessment of viability of intestinal wall in experimental model on rabbits. That is ensured by measuring the blood flow velocity by laser Doppler flowmetry. Additionally, indices of tissue concentration of coenzymes of oxidative metabolism of NADH and FAD of the analyzed region are determined by laser fluorescence spectroscopy. Each index is measured on the analyzed and visually viable intestine sections, determining corresponding differences of measured values and comparing with allowable values, assuming allowable difference of values for blood flow velocity of not more than 4.0910 perfusion units, for coenzyme NADH – not more than – 0.3036 relative units modulus, for co-enzyme FAD – not more than 0.0708 relative units, and evaluating intestinal wall as viable with simultaneous presence of allowable difference values of not less than two of three values.;EFFECT: method allows to simplify, individualize and increase informativity of assessment of intestinal wall viability, increase sensitivity and accuracy of measurements.;1 cl
机译:领域:药物:本发明涉及药物,即涉及腹部器官的外科手术,并且可用于在实验模型中对家兔的肠壁活力进行术中评估。通过使用激光多普勒血流仪测量血流速度来确保这一点。另外,通过激光荧光光谱法确定分析区域的NADH和FAD的氧化代谢的辅酶的组织浓度指数。每个指标均在分析后且在视觉上可行的肠段进行测量,确定相应的测量值差异,并与允许值进行比较,假设血流速度的允许值差异不超过4.0910灌注单位,辅酶NADH –不超过– 0.3036相对单位模量,对于辅酶FAD –不超过0.0708相对单位,并且在同时存在允许的差异值不小于三个值中的两个值的情况下,评估肠壁是否可行。效果:方法可以简化,个性化和增加肠壁生存力评估的信息量,增加测量的灵敏度和准确性。; 1 cl

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