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首页> 外国专利> Method for producing Chinese hamster ovary cell strain, being a producer of recombinant SARS-CoV-2 virus protein RBD, Chinese hamster ovary cell strain, producer of recombinant SARS-CoV-2 protein RBD, method for producing recombinant SARS-CoV-2 virus protein RBD, test system for enzyme immunoassay of human serum or plasma, and use thereof

Method for producing Chinese hamster ovary cell strain, being a producer of recombinant SARS-CoV-2 virus protein RBD, Chinese hamster ovary cell strain, producer of recombinant SARS-CoV-2 protein RBD, method for producing recombinant SARS-CoV-2 virus protein RBD, test system for enzyme immunoassay of human serum or plasma, and use thereof

机译：重组SARS-CoV-2病毒蛋白RBD的生产者的中国仓鼠卵巢细胞株的生产方法，重组SARS-CoV-2蛋白RBD的生产者的中国仓鼠卵巢细胞株，重组SARS-CoV-2病毒的生产方法蛋白质RBD，用于人血清或血浆的酶免疫测定的测试系统及其用途

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FIELD: biotechnology.;SUBSTANCE: The invention relates to the field of biotechnology. Created are a genetic construct for the expression of recombinant SARS-CoV-2 virus protein RBD, a strain of Chinese hamster ovary cells CHO-S-RBD that produces a recombinant protein - a receptor-binding domain (RBD) of SARS-CoV-2 virus, which can be used for diagnostic purposes. Described is a method for producing a strain of Chinese hamster ovary cells CHO-S-RBD being a producer of recombinant SARS-CoV-2 virus protein RBD, containing a genetic construct comprising SEQ ID NO: 1; introducing the specified genetic constructs into cells by lipofection; selecting cells on Hygromycin B antibiotic. Created is a strain of Chinese hamster ovary cells CHO-S-RBD, a producer of recombinant SARS-CoV-2 virus protein RBD. Developed is a method for producing recombinant SARS-CoV-2 virus protein RBD, comprising: culturing a strain of Chinese hamster ovary cells CHO-S-RBD; purifying chromatographically the recombinant SARS-CoV-2 virus protein RBD from culture medium of the Chinese hamster ovary strain CHO-S-RBD; confirming the production of recombinant SARS-CoV-2 protein RBD. Created is a recombinant SARS-CoV-2 virus protein RBD to detect antibodies to SARS-CoV-2. Created is a test system for enzyme immunoassay of human serum or plasma. Developed is a method for analyzing serum or plasma of COVID-19 convalescents to sample the most promising ones for treating patients infected with SARS-CoV-2. Developed is a method for analyzing human serum or plasma to determine the titer of antibodies to SARS-CoV-2 virus. The invention reduces time and simplifies the analysis of serum or plasma of COVID-19 convalescents to sample the most promising ones for treating SARS-CoV-2 infected patients. The invention also provides for high and stable expression of recombinant SARS-CoV-2 virus protein RBD.;EFFECT: invention provides a rapid and efficient test system for determining the most promising serum or plasma samples of COVID-19 convalescents for treating patients infected with SARS-CoV-2.;10 cl, 4 dwg, 6 tbl, 8 ex
机译：技术领域本发明涉及生物技术领域。创建用于表达重组SARS-CoV-2病毒蛋白RBD的基因构建体，RBD是中国仓鼠卵巢细胞CHO-S-RBD的一种菌株，可产生重组蛋白-SARS-CoV-的受体结合域（RBD） 2种病毒，可用于诊断目的。描述了一种生产中国仓鼠卵巢细胞菌株的方法，该菌株是重组SARS-CoV-2病毒蛋白RBD的生产者，其含有包含SEQ ID NO：1的基因构建体;通过脂转染将指定的遗传构建体引入细胞;选择潮霉素B抗生素上的细胞。创建了中国仓鼠卵巢细胞CHO-S-RBD菌株，该菌株是重组SARS-CoV-2病毒蛋白RBD的产生者。开发了一种生产重组SARS-CoV-2病毒蛋白RBD的方法，包括：培养中国仓鼠卵巢细胞CHO-S-RBD株;从中国仓鼠卵巢菌株CHO-S-RBD的培养基中层析纯化重组SARS-CoV-2病毒蛋白RBD。证实了重组SARS-CoV-2蛋白RBD的产生。创建了重组SARS-CoV-2病毒蛋白RBD，以检测针对SARS-CoV-2的抗体。创建用于人血清或血浆酶免疫测定的测试系统。开发了一种用于分析COVID-19康复者的血清或血浆以采样最有前途的方法来治疗SARS-CoV-2感染患者的方法。开发了一种用于分析人血清或血浆以确定SARS-CoV-2病毒抗体滴度的方法。本发明减少了时间并简化了对COVID-19恢复期的血清或血浆的分析，以取样用于治疗SARS-CoV-2感染患者的最有希望的恢复期。本发明还提供了重组SARS-CoV-2病毒蛋白RBD的高而稳定表达。效果：本发明提供了一种快速有效的测试系统，用于确定最有希望的COVID-19疗养期血清或血浆样品，用于治疗感染了COVID-19的患者SARS-CoV-2。; 10 cl，4 dwg，6 tbl，8 ex

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