首页> 外国专利> METHOD OF PRODUCING CYTOGENETIC EPITHELIAL CELL PREPARATIONS FOR CARRYING OUT A FLUORESCENT IN SITU HYBRIDISATION REACTION

METHOD OF PRODUCING CYTOGENETIC EPITHELIAL CELL PREPARATIONS FOR CARRYING OUT A FLUORESCENT IN SITU HYBRIDISATION REACTION

机译:生产用于进行原位杂交反应的细胞遗传上皮细胞制备的方法

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to oncology, and can be used for producing cytogenetic preparations of epithelial cells for carrying out a fluorescent in situ hybridisation (FISH) reaction. That is ensured by epithelial cells slipped off by the cytobrush. They are centrifuged with phosphate-salt buffer for 10 minutes. Then supernatant is removed. A fixing solution consisting of a mixture of methanol and acetic acid in ratio 3:1 is added to the precipitate. Cell suspension is stored at -20 °C. Cytogenetic preparations are prepared by applying cells on zoned slides while monitoring their density. To increase brightness of fluorescent signals, cells are treated with 2 % Carbowax solution for 2–20 hours at 4 °C.;EFFECT: invention prolongs storage of epithelial cell samples and increases brightness of the fluorescent signal during FISH.;1 cl, 2 dwg, 3 ex
机译:技术领域本发明涉及医学,即肿瘤学,并且可以用于产生用于进行荧光原位杂交(FISH)反应的上皮细胞的细胞遗传学制剂。这可以通过上皮细胞被细胞刷滑落来确保。将它们与磷酸盐缓冲液离心10分钟。然后除去上清液。将由比例为3:1的甲醇和乙酸的混合物组成的固定溶液添加到沉淀物中。细胞悬液储存在-20°C下。通过将细胞应用于分区的玻片上,同时监测其密度来制备细胞遗传学制剂。为了增加荧光信号的亮度,将细胞在4°C下用2%的Carbowax溶液处理2-20小时。 dwg,3 ex

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