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PRRS PNA PRRS PNA probe for detecting Porcine reproductive and respiratory syndrome virus genetic type and a method for detecting Porcine reproductive and respiratory syndrome virus genetic type using the same
PRRS PNA PRRS PNA probe for detecting Porcine reproductive and respiratory syndrome virus genetic type and a method for detecting Porcine reproductive and respiratory syndrome virus genetic type using the same
The present invention is a PNA probe represented by the nucleotide sequence of SEQ ID NO: 3 and a PNA probe represented by the nucleotide sequence of SEQ ID NO: 6 with different hybridization patterns with the target nucleic acid according to the porcine reproductive respiratory syndrome virus genotype, and porcine reproductive respiratory syndrome virus using the same The present invention relates to a genotype determination method, and the present invention has the advantage of classifying the porcine reproductive respiratory syndrome virus genotype in one experiment, and accurately determining negative for false-positive results, which were difficult to distinguish easily by conventional methods.
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机译:本发明是由SEQ ID NO:3的核苷酸序列代表的PNA探针,以及由SEQ ID NO:6的核苷酸序列代表的PNA探针,其根据猪繁殖呼吸综合征病毒与靶核酸具有不同的杂交方式。基因型以及使用该基因型的猪生殖道综合症病毒本发明涉及一种基因型确定方法,并且本发明的优点是在一个实验中对猪生殖道综合症病毒基因型进行分类,并准确地确定假阳性结果为阴性。 ,这是传统方法难以区分的。
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