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Method for detecting bubbles between samples using flow cytometry scattering waveform analysis
Method for detecting bubbles between samples using flow cytometry scattering waveform analysis
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机译：流式细胞仪散射波形分析检测样品间气泡的方法
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摘要
Provided herein is a method for detecting separation gas in a fluid flow stream. In one example, the voltage output signal is generated by the scatter detector of the flow cytometer as a flow stream of a plurality of gas-separated samples passes through the flow cytometer. The voltage output signal is sampled, and the timestamp and voltage value are recorded for each sampled voltage of the voltage output signal greater than the separation gap threshold. In some embodiments, the separation gap threshold is at least 2 times greater than the maximum voltage output of the samples. Flow cytometry systems that include software configured to perform these method steps are also described.
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