首页> 外国专利> DETECTION PRIMER AND PNA PROBE CAPABLE OF SCREENING B AND C OF ADENOVIRUS SUBTYPES AND F AND G OF ADENOVIRUS SUBTYPES, AND ADENOVIRUS REAL-TIME MULTIPLEX PCR DETECTION METHOD USING SAME

DETECTION PRIMER AND PNA PROBE CAPABLE OF SCREENING B AND C OF ADENOVIRUS SUBTYPES AND F AND G OF ADENOVIRUS SUBTYPES, AND ADENOVIRUS REAL-TIME MULTIPLEX PCR DETECTION METHOD USING SAME

机译:能够筛查腺病毒亚型的B和C以及腺病毒亚型的F和G的检测底漆和PNA探针,以及使用相同抗原的腺病毒实时多重PCR检测方法

摘要

The present invention relates to a detection composition which extracts nucleic acid of a sample, is amplified using real-time PCR, analyzes a fluorescence signal generated by hybridizing the amplified target nucleic acid with a PNA probe containing a fluorescent substance by temperature, and enables simultaneous and rapid detection of adenoviruses by confirming that the same nucleotide sequence as specific gene markers for each of B, C, F, and G of adenovirus subtypes appears, to a kit, and to a detection method.;COPYRIGHT KIPO 2020
机译:检测组合物技术领域本发明涉及一种检测组合物,其提取样品的核酸,使用实时PCR进行扩增,分析通过将扩增的靶核酸与包含荧光物质的PNA探针在温度下杂交而产生的荧光信号,并且能够同时进行。试剂盒和检测方法,通过确认与腺病毒亚型的B,C,F和G每种特异基因标记相同的核苷酸序列出现,快速检测腺病毒。COPYRIGHTKIPO 2020

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