Manufacturing Method for Cordyceps with Increased Content of Cordycepin and Cordyceps Prepared by the Method

• 1. 一种直接测定冬虫夏草产品中冬虫夏草多糖肽含量的方法 [P] . 中国专利： CN104090038B . 2015.09.30
• 2. 一种直接测定冬虫夏草产品中冬虫夏草多糖肽含量的方法 [P] . 中国专利： CN104090038A . 2014-10-08
• 3. Manufacturing Method for Cordyceps with Increased Content of Cordycepin and Cordyceps Prepared by the Method [P] . 韩国专利： KR20190101792A . 2019-09-02

  机译： 冬虫夏草含量增加的冬虫夏草的制备方法及用该方法制备的冬虫夏草

• 4. Spore suspension comprising insect extract and preparation method of allomyrina dichotoma Cordyceps with increased cordycepin content using this [P] . 韩国专利： KR20200077650A . 2020-07-01

  机译： 含有昆虫提取物的孢子悬浮液和使​​用该虫草素含量增加的双孢冬虫草的制备方法

• 5. Spore suspension comprising insect extract and preparation method of allomyrina dichotoma Cordyceps with increased cordycepin content using this [P] . 韩国专利： KR1020200077650A . 2020.07.01

• 1. Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris [J] . Jian Chen, Hai-Fang Li, Guozhu Zhao, 药物分析学报（英文） . 2021,第004期
• 2. Determination of Cordycepin in Cordyceps kyushuensis by Capillary Electrophoresis and its Antitumour Activity [J] . 中国化学快报（英文版） . 2003,第007期
• 3. Influence of citric acid content on magnetic properties of BaFe12O19 powder prepared by sol-gel auto-combustion method [J] . 钟敏建, 徐国庆, 马洪良, 上海大学学报：英文版 . 2007,第003期
• 4. Culture Conditions for Production of Biomass, Adenosine, and Cordycepin from Cordyceps sinensis CS1197: Optimization by Desirability Function Method [J] . Shashidhar M. Ghatnur, Giridhar Parvatam, Manohar Balaraman Pharmacognosy magazine . 2015,第Suppla3期
• 5. Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology [J] . Li Deng, Tong-Yong Zhou, Li Pi Asian Journal of Chemistry: An International Quarterly Research Journal of Chemistry . 2013,第14期
• 6. A simple and rapid method for identification and determination of cordycepin in Cordyceps militaris by capillary electrophoresis [J] . Rao YK, Chou CH, Tzeng YM Analytica chimica acta . 2006,第2期
• 7. Effects of cooking on the contents of adenosine and cordycepin in Cordyceps militaris [C] . Jiamin Li, Minyi Guan, Yi Li World Congress on Particle Technology . 2015
• 8. Evaluation of methods to increase free procyanidin content in the cider fermentation process of Jonathan Apples. [D] . Tan, Mavis. 2005
• 9. Study on Mix Proportion Optimization of Manufactured Sand RPC and Design Method of Steel Fiber Content under Different Curing Methods [O] . Chunling Zhong, Mo Liu, Yunlong Zhang, 2019
• 10. Quantitative analysis of cordycepin in Cordyceps militaris under different extraction methods [O] . Jungwon Choi, Leo Adrianne Paje, Baekjun Kwon, 2021

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Evaluation of Nutrient Contents of Amaranth Leaves Prepared Using Different Cooking Methods [J] . Olumakaiye M. Funke . 食品与营养科学（英文） . 2011,第4期
• 3. 提取方法对冬虫夏草发酵制品提取液中多种有效成分含量的影响 [C] . 顾宇翔 ,王尊生 ,沈阳师范大学生物系 . 第八届全国工业生化与分子生物学学术大会 . 2005
• 4. 冬虫夏草及虫草制品中活性成分检测方法的建立及含量测定 [A] . 周苏 . 2013