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Nepetalactol redox enzyme, nepetalactol synthase, and microorganisms capable of producing nepetalactone

机译:荆芥内酯氧化还原酶,荆芥内酯合酶和能够产生荆芥内酯的微生物

摘要

The present disclosure provides isolated nepetalactone redox enzyme (NOR) polypeptides, nepetalactol synthase (NEPS), and related polynucleotides, engineered host cells, and cultures, as well as methods of producing NOR and NEPS, and It provides a method of producing nepetalactol, nepetalactone, and dihydronepetalactone using these. In addition, the present disclosure provides a method for producing nepetalactone from a fermentation substrate, such as glucose, by manipulating cells (e.g., microbial cells), as well as engineered cells and related cultures having this ability, and producing nepetalactone. Provides a way.
机译:本公开提供了分离的荆芥内酯氧化还原酶(NOR)多肽,荆芥内酯合酶(NEPS)和相关的多核苷酸,工程改造的宿主细胞和培养物,以及产生NOR和NEPS的方法,并且其提供了荆芥内酯,荆芥内酯的生产方法。 ,以及使用这些的二氢荆芥内酯。另外,本公开提供了一种通过操纵细胞(例如微生物细胞)以及具有这种能力的工程细胞和相关培养物从发酵底物例如葡萄糖产生荆芥内酯的方法,以及生产荆芥内酯的方法。提供了一种方法。

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