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IN VITRO AND IN VIVO BONE METASTASIS METHOD OF PROSTATE CANCER CELLS BY EXPRESSION OF GREEN FLUORESCENT-BOUND CD133 PROTEIN
IN VITRO AND IN VIVO BONE METASTASIS METHOD OF PROSTATE CANCER CELLS BY EXPRESSION OF GREEN FLUORESCENT-BOUND CD133 PROTEIN
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机译：表达绿色荧光结合的CD133蛋白的前列腺癌细胞体外和体内转移方法
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摘要
Green fluorescent-bound proteins have a great influence on research in cell biology. The stem cell theory of cancer proposes that a small number of cancer cell groups are the main reason for tumorigenesis, metastasis, and resistance to anticancer agents. The present patent relates to a method in which a plasmid for expressing GFP-bound CD133 stably induces transformation in prostate cancer cells, to enable monitoring on drug resistance or the like in cancer stem cells through CD133 overexpression. We propose a transfection protocol in which in prostate cancer cell lines PC3 and DU145, CD133 is labeled with GFP (CD133-GFP) to analyze and monitor CD133-GFP-expressed fluorescent expressing cells. In addition, distributions of CD133-GFP-expressed PC3 and DU145 cells are monitored in the intracardiac injected mouse. We propose the procedure for in vivo analysis of tumorigenesis and metastases, following transfection of prostate cancer cell lines PC3 and DU145 and successful transformation. The method of monitoring in vitro and in vivo metastasis of prostate cancer cells to bone via expression of green fluorescent-bound CD133 protein transfects the prostate cancer cell lines with CD133-GFP structure, and can propose a simple and efficient method for CSC research to establish a mouse xenograft model involving intrabile injection of cancer cells.;COPYRIGHT KIPO 2020
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