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DETECTION TECHNOLOGY SYSTEM FOR ENRICHING LOW-ABUNDANCE DNA MUTATION ON THE BASIS OF NUCLEASE-COUPLED PCR PRINCIPLE AND APPLICATION THEREOF
DETECTION TECHNOLOGY SYSTEM FOR ENRICHING LOW-ABUNDANCE DNA MUTATION ON THE BASIS OF NUCLEASE-COUPLED PCR PRINCIPLE AND APPLICATION THEREOF
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机译：基于核酸酶PCR原理富集低丰度DNA突变检测技术系统及其应用
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摘要
A detection system for enriching a low-abundance single-nucleotide mutant gene on the basis of nuclease-coupled PCR principle. Also provided is a method for increasing relative abundance of a target nucleic acid, comprising: (a) providing a nucleic acid sample containing the target nucleic acid and a non-target nucleic acid, the target nucleic acid having abundance of F1a in the nucleic acid sample; and (b) performing PCR and a nucleic acid cleavage reaction in an amplification-cleavage reaction system using the nucleic acids in the nucleic acid sample as templates to obtain an amplification-cleavage reaction product, the target nucleic acid having abundance of F1b in the amplification-cleavage reaction product, and the ratio of F1b/F1a being ≥10.
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