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METHODS AND SYSTEMS FOR SEQUENCING CELL-FREE MICROBIOME DNA

机译:测序无细胞微生物DNA的方法和系统

摘要

The present disclosure provides methods, systems, kits, and compositions for enriching cell-free DNA of a microbiome from a collection of cell-free DNA that includes host cell-free DNA. Such methods and systems may provide for more efficient and economical approaches for analyzing microbiome cell-free DNA by excluding at least some portion of host cell-free DNA from a common collection of cell-free DNA. The microbiome cell-free DNA may be enriched by size selection, by a reduction in the amount of linear DNA, and/or by targeting protein(s) to which host cell-free DNA may be associated.
机译:本公开提供了用于从包括宿主无细胞DNA的无细胞DNA集合中富集微生物组的无细胞DNA的方法,系统,试剂盒和组合物。通过从无细胞DNA的常见集合中排除宿主细胞无DNA的至少某些部分,此类方法和系统可以提供用于分析无微生物组DNA的更有效和经济的方法。可以通过大小选择,通过减少线性DNA的量和/或通过靶向可以与宿主无细胞DNA相关的蛋白质来富集无微生物组无细胞DNA。

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