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DUCK PLAGUE VIRUS GE AND GI DUAL-GENE TRACELESS DELETION STRAIN DPV CHV-DELTA GE + DELTA GI AND CONSTRUCTION METHOD THEREFOR
DUCK PLAGUE VIRUS GE AND GI DUAL-GENE TRACELESS DELETION STRAIN DPV CHV-DELTA GE + DELTA GI AND CONSTRUCTION METHOD THEREFOR
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机译:鸭瘟病毒GE和GI双代无痕追踪菌株DPV CHV-DELTA GE + DEL GI及其构建方法
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摘要
Provided are a duck plague virus gE and gI dual-gene traceless deletion strain DPV CHv-delta gE + delta gI and a construction method therefor. A duck plague virus gE gene and a duck plague virus gI gene are deleted by means of two times of homologous recombination on a bacterial artificial chromosome recombination duck plague virus rescue system platform by using a GS1783 escherichia coli strain and a pEPkan-S plasmid, a MiniF element is deleted by using an intracellular spontaneous homologous recombination method, and thus the construction of a duck plague virus dual-gene traceless deletion strain free from exogenous base and MiniF element residue is completed for the first time.
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机译:提供了鸭瘟病毒gE和gI双基因无痕缺失株DPV CHv-delta gE + delta gI及其构建方法。通过使用GS1783大肠杆菌菌株和pEPkan-S质粒在细菌人工染色体重组鸭瘟病毒拯救系统平台上进行两次同源重组来删除鸭瘟病毒gE基因和鸭瘟病毒gI基因。通过细胞内自发同源重组方法缺失MiniF元件,从而首次完成了无外源碱基和MiniF元件残基的鸭瘟病毒双基因无痕缺失菌株的构建。
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