首页>
外国专利>
A method for constructing a high-throughput cotton variety DNA fingerprint library based on a capillary four-color fluorescence electrophoresis detection system and multiplex fluorescence PCR amplification
A method for constructing a high-throughput cotton variety DNA fingerprint library based on a capillary four-color fluorescence electrophoresis detection system and multiplex fluorescence PCR amplification
展开▼
机译：基于毛细管四色荧光电泳检测系统和多重荧光PCR扩增的高通量棉花品种DNA指纹图谱构建方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
#$%^&*AU2020101755A420200917.pdf#####ABSTRACT The invention discloses a method for constructing a high-throughput cotton variety DNA fingerprint library based on a capillary four-color fluorescence electrophoresis detection system and multiplex fluorescence PCR amplification, which comprises the following steps: carrying out multiplex fluorescence PCR combination on 52 pairs of core primers distributed on 26 chromosomes of the whole genome of cotton subgroup A or D, extracting cotton DNA as a sample to be detected. Five combinations obtained by combining 52 pairs of core primers according to multiple fluorescent PCR combinations were used to carry out pSSR-PCR amplification reaction on the sample to be tested to obtain PCR amplification products, and the PCR amplification products were detected by capillary four-color fluorescent electrophoresis and data analysis. The method can greatly improve the detection flux and has high detection efficiency, and is especially suitable for the construction of DNA fingerprint database of a large number of cotton varieties.
展开▼
