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A method for detecting polyamine contents in goose tissues by high performance liquid chromatography
A method for detecting polyamine contents in goose tissues by high performance liquid chromatography
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机译：高效液相色谱法检测鹅组织中多胺含量的方法
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#$%^&*AU2020101754A420200917.pdf#####ABSTRACT The invention discloses a method for detecting polyamine (putrescine, spermidine and spermine) contents in goose tissues by high performance liquid chromatography, which comprises the following chromatographic conditions: C18 chromatographic column (5 m, 4.6x250 mm); The proportion of mobile phases (methyl alcohol:water volume ratio) is 62:38; The flow rate is 1.0 mL/min; Ultraviolet detection wavelength is 229 nm; Column temperature is 25°C. Tissue solid phase extraction method: 0.1 g tissue is added into 1 mL 5% HC104 and 10 L internal standard, and the mixture is homogenized and exacted the supernatant. And 2 mL 2.5 mol/mL NaOH and 1.4 [L benzoyl chloride is added and bathed in water at 40°C for 30 min. The pH value of the mixture is adjusted to 7.0, added into C18 column, washed with 15 mL water and 15 mL 15% methanol, and eluted with 0.5 L methanol. This scheme can effectively separate putrescine, spermidine and spermine from tissues, and can quickly and accurately detect polyamine contents in goose tissues with simple operation, high accuracy and good repeatability.-1/6Internal Standard 35 302520- Putrescine 15 10 0 2.5 5 7.5 10 12.5 15 Min Fig.1I
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机译：＃$％^＆* AU2020101754A420200917.pdf #####抽象本发明公开了一种检测多胺（腐胺，亚精胺）的方法。高效液相色谱法测定鹅组织中的精胺含量）其中包括以下色谱条件：C18色谱柱（5 m，4.6x250 mm）;流动相的比例（甲醇：水体积比）为62:38;流速为1.0 mL / min;紫外线检测波长是229 nm;柱温为25°C。组织固相萃取方法：0.1 g将组织加入1 mL 5％HC104和10 L内标物中，将混合物匀浆并上清。 2 mL 2.5 mol / mL NaOH和1.4 [L加入苯甲酰氯并在40℃的水中浴30分钟。的pH值将混合物调节至7.0，加入C18柱，用15 mL水和15mL 15％甲醇，并用0.5 L甲醇洗脱。这个方案可以有效从组织中分离出腐胺，亚精胺和亚精胺，并且可以快速和操作简单，准确度高，可准确检测鹅组织中的多胺含量准确性和良好的重复性。-1/6内部标准35<302520-腐胺15100 2.5 5 7.5 10 12.5 15分钟图1I
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