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RECESSIVE RESISTANCE TOBACCO GENE EIFISO4E-S OF TOBACCO VEIN BANDING MOSAIC VIRUS AND APPLICATION THEREOF
RECESSIVE RESISTANCE TOBACCO GENE EIFISO4E-S OF TOBACCO VEIN BANDING MOSAIC VIRUS AND APPLICATION THEREOF
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机译:烟草带状烟草毒力的烟草抗性基因Eifiso4E-S及其应用
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摘要
An isolation cloning and breeding application of a recessive resistance gene eIFiso4E-S of a tobacco vein banding mosaic virus (TVBMV). The nucleotide sequence of the tobacco recessive resistance gene eIFiso4E-S of the TVBMV is as shown in SEQ ID NO.1, and the amino acid of an encoded polypeptide thereof is as shown in SEQ ID NO.2; the gene in the tobacco is knocked out to obtain an eIFiso4E-SKO material; and the eIFiso4E-SKO is polymerized with a va or eIF4E1KO gene of potyvirus (PVY), thereby enabling the tobacco to be resistant to TVBMV and PVY.
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机译:烟草静脉带状花叶病毒(TVBMV)的隐性抗性基因eIFiso4E-S的分离克隆和育种应用。 TVBMV的烟草隐性抗性基因eIFiso4E-S的核苷酸序列如SEQ ID NO.1所示,其编码多肽的氨基酸如SEQ ID NO.2所示。敲除烟草中的基因以获得eIFiso4E-S KO Sup>材料。并将eIFiso4E-S KO Sup>与马铃薯Y病毒(PVY)的va或eIF4E1 KO Sup>基因聚合,从而使烟草对TVBMV和PVY具有抗性。
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