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首页> 外国专利> SUS SCROFA VACCINE VIRUS AGAINST AFRICAN SWINE FEVER (ARMENIA/07) AND GENOTYPE II DERIVATIVES)

SUS SCROFA VACCINE VIRUS AGAINST AFRICAN SWINE FEVER (ARMENIA/07) AND GENOTYPE II DERIVATIVES)

机译：抗非洲猪瘟的 SUS SCROFA 疫苗病毒（亚美尼亚/ 07）和基因型II衍生物

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A Sus scrofa vaccine virus against African swine fever (Armenia/07) and genotype II derivatives). A field isolate of ASFV known as Lv17/WB/Rie1 is obtained and characterised in this invention. The Sus scrofa vaccine virus strain against African swine fever (Armenia/07 and genotype II derivatives) was isolated from a wild boar hunted in Rietumpieriga (Engures, Smardesu), Latvia, in February 2017. The virus has been characterised as the first non-haemoadsorbent (no-HAD) ASFV isolate from genotype II. The nucleotide sequence for the gene EP402R in the Lv17/WB/Rie1 vaccine isolate responsible for the HAD phenomenon contains a mutation in the reading frame. This modification is not present in HAD viruses from genotype II. The use of molecular techniques with specific primers for that full sequence will allow vaccinated and unvaccinated animals to be differentiated. In addition, the vaccine tests carried out show that this vaccine induces a very high level of protection, specifically 92.86%, against the highly virulent Armenia/07 isolate. Finally, the in vitro growth characteristics of the vaccine isolate Lv17/WB/Rie1 have been evaluated by culturing same in primary porcine peripheral blood monocyte (PBM) cultures obtained from the peripheral blood of domestic pigs. After a first pass of the vaccine virus in cell culture, the virus was collected to create a stock of Lv17/WB/Rie vaccine virus.
机译：一种针对非洲猪瘟（亚美尼亚/ 07）和基因型II衍生物的 Sscrofa 疫苗病毒。获得了被称为Lv17 / WB / Rie1的ASSF的现场分离株并在本发明中表征。于2017年2月从拉脱维亚Rietumpieriga（Engures，Smardesu）猎获的野猪中分离出了针对非洲猪瘟的（I> Sus scrofa 疫苗病毒株（亚美尼亚/ 07和基因型II衍生物）。被表征为第一个来自基因型II的非溶血性ASFV分离株。负责HAD现象的Lv17 / WB / Rie1疫苗分离物中EP402R基因的核苷酸序列在阅读框中含有突变。在基因型II的HAD病毒中不存在这种修饰。将分子技术与针对该完整序列的特定引物结合使用，将使已接种疫苗和未接种疫苗的动物得以区分。另外，进行的疫苗测试表明，该疫苗对高毒力的亚美尼亚/ 07分离株具有很高的保护水平，特别是92.86％。最后，通过在从家猪外周血获得的原代猪外周血单核细胞（PBM）培养物中培养疫苗分离株Lv17 / WB / Rie1的体外生长特性进行了评估。疫苗病毒在细胞培养物中首次通过后，收集该病毒以创建Lv17 / WB / Rie疫苗病毒库。

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