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REAL-TIME FLUORESCENT QUANTITATIVE PCR DETECTION METHOD BASED ON RUTHENIUM COMPLEX AND KIT
REAL-TIME FLUORESCENT QUANTITATIVE PCR DETECTION METHOD BASED ON RUTHENIUM COMPLEX AND KIT
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机译：基于钌配合物和试剂盒的荧光定量PCR实时检测方法
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摘要
Disclosed are a real-time fluorescent quantitative PCR detection method based on a ruthenium complex and a kit. The present invention establishes a real-time fluorescent quantitative PCR detection method with a ruthenium complex as a fluorescent dye. The results show that the ruthenium complex, as a fluorescent dye, can be used within a wider concentration range, and a melting curve can be generated for specific amplification verification and multiple PCR detection.
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