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LINEAGE TRACING USING MITOCHONDRIAL GENOME MUTATIONS AND SINGLE CELL GENOMICS

机译：利用线粒体基因突变和单细胞基因组进行谱系追踪

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摘要

Embodiments disclosed herein provide methods of using somatic mutations in mitochondrial genomes to retrospectively infer cell lineages in native contexts and to serve as genetic barcodes to measure clonal dynamics in complex cellular populations.Further, somatic mutations in mitochondrial DNA (mtDNA) are tracked by single cell genomic approaches for simultaneous analysis of single cell lineage and state. Applicants further show that mitochondrial mutations can be readily detected with contemporary single cell transcriptomic and epigenomic technologies to concomitantly capture gene expression profiles and chromatin accessibility, respectively.

机译：本文公开的实施方案提供了使用线粒体基因组中的体细胞突变在自然环境中回顾性推断细胞谱系并用作遗传条形码以测量复杂细胞群体中的克隆动态的方法。同时分析单个细胞谱系和状态的基因组方法。申请人进一步表明，利用当代的单细胞转录组学和表观基因组学技术可以很容易地检测线粒体突变，以分别捕获基因表达谱和染色质可及性。

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公开/公告号WO2019241273A1

专利类型
公开/公告日2019-12-19

原文格式PDF
申请/专利权人 THE BROAD INSTITUTE INC.;MASSACHUSETTS INSTITUTE OF TECHNOLOGY;THE CHILDREN’S MEDICAL CENTER CORPORATION;PRESIDENT AND FELLOWS OF HARVARD COLLEGE;ULIRSCH JACOB C.;
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申请/专利号WO2019US36583
发明设计人 REGEV AVIV;LAREAU CALEB A.;LUDWIG LEIF S.;SANKARAN VIJAY G.;BUENROSTRO JASON;
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申请日2019-06-11
分类号C12Q1/6809;C12Q1/6881;C12Q1/6886;
国家 WO
入库时间 2022-08-21 11:14:11

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