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首页> 外国专利> TECHNOLOGY FOR PRODUCING PHYTOCOMPLEXES WITH HIGH CONCENTRATION OF ISOFLAVONOIDS FROM TRIFOLIUM PRATENSE (), BASED ON SEPARATIVE, SELECTIVE AND GUIDED PROCESSES

TECHNOLOGY FOR PRODUCING PHYTOCOMPLEXES WITH HIGH CONCENTRATION OF ISOFLAVONOIDS FROM TRIFOLIUM PRATENSE (), BASED ON SEPARATIVE, SELECTIVE AND GUIDED PROCESSES

机译：基于分离，选择和指导过程的由三叶草（）制备高浓度异黄酮类化合物的植物复合物的技术

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The invention relates to a process for producing a phytocomplex with high isoflavonoid concentration from Trifolium pratense to be used as an anti-hyperglycemic agent. According to the invention, the process consists of the following steps : the primary processing of the plant material, the preparation of a 10% hydro-alcoholic extract in 50% ethanol by ultrasonication for 1 h at a frequency of 35 kHz, the percentage being expressed by mass, the pre-filtration, followed by microfiltration through membranes with a pore size of 0.22 … 0.45 μ m, at a pressure of 2 ... 4 bar, the concentration by ultra-filtration on ultra-filtration membranes with cut-off of 1000 ... 3000 Da, at a pressure of 8 …9 bar, resulting in a phytocomplex having a total polyphenol content of 1542 ... 1550 mg/l, expressed as gallic acid, and a flavone content of 896 ... 900 mg/l, expressed as rutin, exhibiting an α -amylase enzyme inhibitory activity higher than 60% and an α -glucosidase enzyme inhibitory activity higher than 90%.
机译：本发明涉及从三叶草（Trifolium pratense）生产具有高异黄酮浓度的植物复合物的方法，该植物复合物用作抗降血糖药。根据本发明，该方法包括以下步骤：植物材料的初级加工，在35 kHz的频率下超声处理1 h，在50％的乙醇中制备10％的水醇提取物。用质量表示，预过滤，然后在2 ... 4 bar的压力下，通过孔径为0.22…0.45μm的膜进行微滤，并在具有截留值的超滤膜上进行超滤浓缩在8…9 bar的压力下从1000 ... 3000 Da中分离出来，得到的植物复合物的总多酚含量为1542 ... 1550 mg / l，表示为没食子酸，黄酮含量为896 .. 900mg / l，以芦丁表示，表现出高于60％的α-淀粉酶抑制活性和高于90％的α-葡糖苷酶抑制活性。

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