The invention relates to a method for binding enzymes of sarcosine oxidase and horseradish peroxidase to FeO/Au nanoparticles by chitosan for qualitative and quantitative determination of sarcosine in a biological or environmental sample using these nanoparticles modified by the enzymes with the evaluation of a reaction in a solution or on a diagnostic strip. The test is suitable for routine determination of sarcosine in an amount of 1 to 100 µg/ml in a biological sample, especially urine with high sensitivity. The determination is feasible within 0.5 to 1 hour using commonly available equipment.
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