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Nucleotide sequence of synthetic Cas9 gene, a cassette directing sgRNA for editing of plant genome and the efficient system for side-directed mutagenesis of a selected plant genome

机译:合成Cas9基因的核苷酸序列,指导sgRNA编码植物基因组的盒以及对选定植物基因组进行侧向诱变的有效系统

摘要

The subject of the notification is a synthetic Cas9 endonucleus gene with a nucleotide sequence presented as sequence number one. The notification shall also include an empty SSR cassette and a completed SSR cassette. In addition, a set for the production of the Cas9 /sgRNA system and the method of editing the single -leaf genome, in particular barley, is also covered by the notification.
机译:通知的主题是一个合成的Cas9核内基因,其核苷酸序列为第一个序列。该通知还应包括一个空的SSR盒带和一个完整的SSR盒带。此外,该通知还包括用于生产Cas9 / sgRNA系统的套件以及编辑单叶基因组(特别是大麦)的方法。

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