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Nucleic Acid and Kit for Simultaneously detecting Pathogens Causing Damping Off, Spot Disease and Powdery Mildew on Begonia
Nucleic Acid and Kit for Simultaneously detecting Pathogens Causing Damping Off, Spot Disease and Powdery Mildew on Begonia
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机译：核酸和试剂盒可同时检测导致秋海棠减毒，斑点病和白粉病的病原体
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摘要
The present invention relates to a group of nucleic acids, a kit and a detection method for simultaneously detecting three pathogens causing damping off, spot disease and powdery mildew on Begonia. The nucleic acids used for multipleX PCR to simultaneously detect three pathogens include upstream and downstream primers of three pathogens causing damping off, spot disease and powdery mildew on Begonia. The nucleic acids used for multipleX PCR to simultaneously detect three pathogens further include probes corresponding to each pathogen. The present invention further establishes a relatively efficient and sensitive detection method for simultaneously detecting the three pathogens causing damping off, spot disease and powdery mildew on Begonia. The method can effectively improve the sensitivity of detection, avoid the occurrence of false negative results, and provides a technical means for effectively preventing the occurrence of damping off, spot disease and powdery mildew on Begonia.
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