首页> 外国专利> CHIMERIC TRANSCRIPTION FACTOR VARIANTS WITH AUGMENTED SENSITIVITY TO DRUG LIGAND INDUCTION OF TRANSGENE EXPRESSION IN MAMMALIAN CELLS.

CHIMERIC TRANSCRIPTION FACTOR VARIANTS WITH AUGMENTED SENSITIVITY TO DRUG LIGAND INDUCTION OF TRANSGENE EXPRESSION IN MAMMALIAN CELLS.

机译:嵌合转录因子变体对哺乳动物细胞中转基因表达的药物配体诱导敏感性增强。

摘要

Provided herein is a system for inducible expression of a chimeric antigen receptor in cells. The system comprises: a) a first nucleic acid comprising a first promoter inducible by a drug, wherein the first nucleic acid is operably linked to a first polynucleotide that encodes a chimeric antigen receptor, which comprises a ligand binding domain, a second polynucleotide, which encodes a spacer, a third polynucleotide, which encodes a transmembrane domain and a fourth polynucleotide, which encodes an intracellular signaling domain, and b) a second nucleic acid comprising a second promoter that is operably linked to a nucleic acid encoding a transcriptional activator for the first promoter inducible by drug. Methods of making such cells and methods of treatment using these cells are also provided.
机译:本文提供了用于在细胞中诱导表达嵌合抗原受体的系统。该系统包括:a)第一核酸,其包含可被药物诱导的第一启动子,其中所述第一核酸可操作地连接至编码嵌合抗原受体的第一多核苷酸,所述第一多核苷酸包含配体结合结构域,第二多核苷酸,编码间隔子,编码跨膜结构域的第三多核苷酸和编码细胞内信号传导结构域的第四多核苷酸,以及b)包含第二启动子的第二核酸,该第二启动子可操作地连接至编码该蛋白的转录激活子的核酸药物诱导的第一个启动子。还提供了制备此类细胞的方法和使用这些细胞的治疗方法。

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