首页> 外国专利> METHOD FOR PROMOTING ENZYMOLYSIS OF LIGNOCELLULOSE BY USING PH-RESPONSIVE LIGNIN AMPHOTERIC SURFACTANT AND RECOVERY OF CELLULASE

METHOD FOR PROMOTING ENZYMOLYSIS OF LIGNOCELLULOSE BY USING PH-RESPONSIVE LIGNIN AMPHOTERIC SURFACTANT AND RECOVERY OF CELLULASE

机译:PH响应性木质素两性表面活性剂促进纤维素酶酶解和纤维素酶回收的方法

摘要

A method for promoting enzymolysis of lignocellulose by using a pH-responsive lignin amphoteric surfactant and recovery of a cellulase. The method includes: adding lignocellulose to a buffer solution, and then adding a pH-responsive lignin amphoteric surfactant and a cellulase; controlling the pH of the mixed solution to be 4.0-6.2, heating the solution to a temperature of 40° C. to 60° C. and reacting at said temperature for 24-96 h to obtain a saccharified hydrolyzate of lignocellulose; and obtaining an enzymolysed liquid by solid-liquid separation, and then adjusting the pH of the enzymolysed liquid to precipitate the pH-responsive lignin amphoteric surfactant and the cellulase for recycling. The method can effectively improve the enzymolysis efficiency of lignocellulose, recover a certain amount of cellulase, and expand the applications of industrial lignin. The method does not require additional equipment, and is simple to operate and environmentally friendly.
机译:一种通过使用pH响应性木质素两性表面活性剂促进木质纤维素的酶水解并回收纤维素酶的方法。该方法包括:将木质纤维素添加到缓冲溶液中,然后添加pH响应性木质素两性表面活性剂和纤维素酶。将混合溶液的pH控制为4.0-6.2,加热至40℃至60℃,并在该温度下反应24-96h,得到木质纤维素的糖化水解产物。通过固液分离得到酶解液,然后调节酶解液的pH以沉淀pH响应性木质素两性表面活性剂和纤维素酶以进行回收。该方法可有效提高木质纤维素的酶解效率,回收一定量的纤维素酶,扩大工业木质素的应用范围。该方法不需要额外的设备,并且操作简单并且对环境友好。

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