首页>
外国专利>
Buffer-and-salt-content-independent UV-VIS spectroscopic characterisation of sample DNA and/or RNA content using reference samples having distinct buffer and/or salt content
Buffer-and-salt-content-independent UV-VIS spectroscopic characterisation of sample DNA and/or RNA content using reference samples having distinct buffer and/or salt content
A method is described for characterising a sample comprising at least DNA and/or RNA. The method comprises obtaining a UV-VIS spectroscopic measurement of the sample comprising at least DNA and/or RNA, deriving an absorption parameter based on the UV-VIS spectroscopic measurement, and determining a DNA and/or RNA quantification present in the sample. Determining a DNA and/or RNA quantification thereby comprises taking into account a buffer and/or salt effect on the UV-VIS spectroscopic measurement irrespective of the actual buffer and salt content of the sample, based on said derived absorption parameter and reference spectral data including spectral information for buffer and/or salt effects.
展开▼
