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Dual mass spectrometry-cleavable crosslinking reagents for protein-protein interactions

机译:用于蛋白质相互作用的双重质谱可裂解交联剂

摘要

Described are dual mass-spectrometry-cleavable cross-linkers that can be cleaved selectively using two differential tandem mass-spectrometric techniques such as collision induced dissociation (CID) or electron transfer dissociation (ETD), i.e., a dual cleavable crosslinking technology (DUCCT) cross-linker. When used to cross-link a macromolecule, such as a peptide, MS/MS fragmentation produces two signature complementary mass spectra of same cross-linked peptides, the analysis of which gives rise to high confidence in characterizing the structures of the cross-linked macromolecules as well as sites of interactions. Also described, are methods of making and using DUCCT cross-linkers.
机译:描述了可以使用两种差分串联质谱技术(例如碰撞诱导解离(CID)或电子转移解离(ETD))即双重可裂解交联技术(DUCCT)选择性裂解的双重质谱可裂解交联剂。交联剂。当用于交联大分子(例如肽)时,MS / MS片段化会产生两个相同交联肽的特征性互补质谱图,对其进行分析可高度表征交联大分子的结构以及互动场所。还描述了制备和使用DUCCT交联剂的方法。

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