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Dual mass spectrometry-cleavable crosslinking reagents for protein-protein interactions
Dual mass spectrometry-cleavable crosslinking reagents for protein-protein interactions
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机译:用于蛋白质相互作用的双重质谱可裂解交联剂
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摘要
Described are dual mass-spectrometry-cleavable cross-linkers that can be cleaved selectively using two differential tandem mass-spectrometric techniques such as collision induced dissociation (CID) or electron transfer dissociation (ETD), i.e., a dual cleavable crosslinking technology (DUCCT) cross-linker. When used to cross-link a macromolecule, such as a peptide, MS/MS fragmentation produces two signature complementary mass spectra of same cross-linked peptides, the analysis of which gives rise to high confidence in characterizing the structures of the cross-linked macromolecules as well as sites of interactions. Also described, are methods of making and using DUCCT cross-linkers.
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