Method for producing high lycopene ketchup, high lycopene ketchup, and method for increasing lycopene concentration and suppressing viscosity increase

• 1. 番茄酱制取结晶番茄红素及/或番茄红素油树脂的方法 [P] . 中国专利： CN1121455C . 2003.09.17
• 2. 番茄酱制取结晶番茄红素及/或番茄红素油树脂的方法 [P] . 中国专利： CN1298904A . 2001-06-13
• 3. Method for producing high lycopene ketchup, high lycopene ketchup, and method for suppressing high lycopene concentration and increase in viscosity [P] . 日本专利： JP2020080746A . 2020.06.04
• 4. Method for producing high lycopene ketchup, high lycopene ketchup, and method for achieving both high lycopene concentration and suppression of increase in viscosity [P] . 日本专利： JP6736238B2 . 2020-08-05

  机译： 高番茄红素番茄酱的生产方法，高番茄红素番茄酱以及实现高番茄红素浓度和抑制粘度增加的方法

• 5. Method for producing high lycopene ketchup, high lycopene ketchup, and method for achieving both high lycopene concentration and suppression of increase in viscosity [P] . 日本专利： JP2020099281A . 2020.07.02

• 1. Two Lycopene β-Cyclases Genes from Sweet Orange （Citrus sinensis L. Osbeck） Encode Enzymes With Different Functional Efficiency During the Conversion of Lycopene-to-Provitamin A [J] . ZHANG Jian-cheng, ZHOU Wen-jing, XU Qiang, 农业科学学报：英文版 . 2013,第010期
• 2. Two Lycopene β-Cyclases Genes from Sweet Orange (Citrus sinensis L. Osbeck) Encode Enzymes With Different Functional Efifciency During the Conversion of Lycopene-to-Provitamin A [J] . ZHANG Jian-cheng, ZHOU Wen-jing, XU Qiang, 农业科学学报（英文版） . 2013,第010期
• 3. Chronic ingestion of lycopene-rich tomato juice or lycopene supplements significantly increases plasma concentrations of lycopene and related tomato carotenoids in humans. [J] . Paetau I, Khachik F, Brown ED, The American Journal of Clinical Nutrition: Official Journal of the American Society for Clinical Nutrition . 1998,第6期
• 4. Loss of Carotene-9′,10’-Monooxygenase Expression Increases Serum and Tissue Lycopene Concentrations in Lycopene-Fed Mice [J] . Nikki A. Ford4, Steven K. Clinton5, Johannes von Lintig6, The Journal of Nutrition: Official Organ of the American Institute of Nutrition . 2010,第12期
• 5. Consumption of canned citrus fruit meals increases human plasma beta-cryptoxanthin concentration, whereas lycopene and beta-carotene concentrations did not change in healthy adults [J] . Zhu Chenghao H., Gertz Erik R., Cai Yimeng, Nutrition Research . 2016,第7期
• 6. Increase of the Lycopene Production in the Recombinant Strains of Escherichia coli by Supplementing with Fructose [C] . Tong-Cun Zhang, Wen Li, Xue-Gang Luo, International conference on applied biotechnology . 2015
• 7. Coordinated Transcriptional Regulation of the Carotenoid Biosynthesis Pathway Genes Contributes to Fruit Lycopene Content in High-Lycopene Tomato Breeding Cultivars [D] . Duduit, James Robert. 2020
• 8. Loss of Carotene-9′10’-Monooxygenase Expression Increases Serum and Tissue Lycopene Concentrations in Lycopene-Fed Mice [O] . Nikki A. Ford, Steven K. Clinton, Johannes von Lintig, -1
• 9. Chronic ingestion of lycopene-rich tomato juice or lycopene supplements significantly increases plasma concentrations of lycopene and related tomato carotenoids in humans [O] . I Paetau, F Khachik, E D Brown, 1998

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Effects of Ca^(2+)/CaM Messenger System Inhibitors on Ethylene-Induced Increase in Lycopene Content [J] . GUCai-qin ,XIYu-fang ,GUANJun-feng . 中国农业科学：英文版 . 2003,第12期
• 3. Lycopene Concentration and Physico-Chemical Properties of Tropical Fruits [J] . Luis Eduardo Ordonez-Santos ,Diana Patricia Ledezma-Realpe . 食品与营养科学（英文） . 2013,第7期
• 4. 高番茄红素含量中小果型西瓜新品种绿野无籽的选育 [C] . 刘文革 ,阎志红 ,赵胜杰 . 第十五次全国无籽西瓜科研与生产协作组会议 . 2014
• 5. 高番茄红素性状的遗传分析及分子标记 [A] . 严玉坤 . 2009