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Methods for single-cell protein expression profiling of bottom plate midbrain dopaminergic progenitor cells
Methods for single-cell protein expression profiling of bottom plate midbrain dopaminergic progenitor cells
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机译:底板中脑多巴胺能祖细胞单细胞蛋白表达谱的方法
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摘要
The present invention is an in vitro method for analyzing a cell composition containing human bottom plate mesDA precursor cells, a) converting cells of the cell composition or cells of a sample thereof to antigens FOXA2, OTX2, PAX6, and NKX6.1. In the step of labeling the cells of the cell composition or the sample by contact with a specific antigen-binding molecule, b) in the step of determining the percentage of the cells labeled with the antigen-binding molecule for each of the antigens. In the protein expression profile of the cells, 80-100% of the cells are positive for FOXA2, 80-100% of the cells are positive for OTX2, less than 10% of the cells are positive for PAX6, and the cells Provided is a method comprising the step of eligifying cells of the cell composition as human bottom plate mesDA precursor cells if less than 10% are positive for NKX 6.1. A kit containing the antigen-binding molecule for use in the method is also provided.
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