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METHOD FOR DETERMINING WATER SOLUBLE PROTEIN CONTENT (WSPC) IN SOYBEAN BY NEAR INFRARED SPECTROSCOPY (NIRS)
METHOD FOR DETERMINING WATER SOLUBLE PROTEIN CONTENT (WSPC) IN SOYBEAN BY NEAR INFRARED SPECTROSCOPY (NIRS)
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机译:近红外光谱法测定大豆中水溶性蛋白含量的方法
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摘要
The present invention provides a method for determining a water soluble protein content (WSPC) in soybean by NIRS, and relates to the field of near infrared spectroscopy (NIRS). The method according to the present invention includes peeling off seed coat of to-be-tested soybean, and scanning the peeled soybean with a near infrared spectrometer to obtain an original spectrum, and substituting the original spectrum of the soybean into a model for predicting a WSPC in soybean to obtain the WSPC in soybean, where when the model for predicting a WSPC in soybean is constructed, the soybean is peeled, defatted and determined by a chemical method, the original spectrum is preprocessed by a plurality of methods, and a measured value and a predicted value are compared to evaluate a quantitative analysis model obtained by a PLS regression method. This improves the accuracy of the model for predicting a WSPC in soybean. The method according to the present invention solves a measurement error caused by a seed coat color difference. When the model for predicting a WSPC in soybean is constructed, the peeled soybean is defatted first, and the influence of fat particles on water soluble protein is removed, thereby obtaining a more accurate prediction model.
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