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FLUORESCENT PROBE, FLUORESCENCE DETECTION METHOD, AND METHOD FOR USING FLUORESCENT PROBE
FLUORESCENT PROBE, FLUORESCENCE DETECTION METHOD, AND METHOD FOR USING FLUORESCENT PROBE
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机译：荧光探针，荧光检测方法以及使用荧光探针的方法
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摘要
The present invention relates to a fluorescent probe including a carrier molecule, a fluorescent dye a bound to the carrier molecule, and a fluorescent dye b bound to the carrier molecule in which the excitation wavelengths of the fluorescent dyes a and b are different, and FRET does not occur between the fluorescent dyes a and b. The present invention also relates to a method for detecting fluorescence that includes a step of labeling target cells with the fluorescent probe, and a step of irradiating the target cells labeled with the fluorescent probe with excitation light and observing the fluorescence from the fluorescent probe. The present invention also relates to a method for using a fluorescent probe that includes a step of fluorescently labeling cells with the fluorescent probe, a step of screening the fluorescence-labeled cells using a flow cytometer or a fluorescence microscope, a step of transplanting the screened fluorescence-labeled cells into a living organism, and a step of observing the fluorescence-labeled cells in the living organism using an in vivo fluorescence imaging apparatus.
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