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METHOD FOR TOTAL GLYCEROL ANALYSIS OF A BIODIESEL SAMPLE BASED ON SPECTROPHOTOMETRY DETECTION

机译:分光光度法测定生物样品中总甘油含量的方法

摘要

The invention relates to a method for analyzing a biofuel sample comprising methyl esters of fatty acids for determining the total glycerol content, characterized in that: the solid phase extraction of the free glycerol and the esters derived from glycerol is carried out from the biodiesel sample in a column containing a stationary phase of aminopropylsilane, and then, on the one hand, the methyl esters of fatty acids and, on the other hand, the phase containing the free glycerol and its derivatives, and then separating the phase containing the glycerol and its derivatives and mixing it with an enzymatic kit so as to obtain a colored compound (CC) and, said colored compound is analyzed to measure the concentration of total glycerol.
机译:本发明涉及一种用于分析包含脂肪酸甲酯的生物燃料样品的方法,以确定总甘油含量,其特征在于:游离甘油和衍生自甘油的酯的固相萃取是从生物柴油样品中进行的。含有氨基丙基硅烷固定相的色谱柱,一方面是脂肪酸的甲酯,另一方面是含有游离甘油及其衍生物的相,然后将含有甘油及其衍生物的相分离衍生物并将其与酶试剂盒混合以获得有色化合物(CC),然后分析所述有色化合物以测量总甘油的浓度。

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