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Optimized host / vector system for the generation of protective mono- and multivalent subunit vaccines based on the yeast Kluyveromyces lactis

机译:优化的宿主/载体系统,用于产生基于酵母克鲁维酵母的保护性单价和多价亚基疫苗

摘要

The invention relates to recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for the generation of a protective immune response against pathogens. In particular, the invention provides K. lactis strains for the targeted cloning of foreign antigen encoding nucleic acids into the yeast genome of the K. lactis strain, characterized in that the K. lactis strain is used alternatively or in addition to the KILAC4 locus at the KIURA3-20 locus ( KLLA0E22771g) and / or at the KIMET5-1 locus (KLLA0B03938g) has integrated expression cassettes for foreign antigens. The invention further relates to integrative expression vectors and methods for producing the K. lactis strains of the invention and their use as vaccines.
机译:本发明涉及能够高效表达一种或多种外源蛋白质并适于用作疫苗以产生针对病原体的保护性免疫应答的重组克鲁维酵母(K.lactis)酵母。特别地,本发明提供了乳酸克鲁维酵母菌株,用于将外源抗原编码核酸靶向克隆到乳酸克鲁维酵母菌株的酵母基因组中,其特征在于,所述乳酸克鲁维酵母菌株可替代地或除了在KILAC4基因座外使用。 KIURA3-20位点(KLLA0E22771g)和/或KIMET5-1位点(KLLA0B03938g)具有整合的外源抗原表达盒。本发明进一步涉及整合表达载体和产生本发明乳酸克鲁维酵母菌株的方法及其作为疫苗的用途。

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