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首页> 外国专利> SERUM-FREE NUTRIENT MEDIUM FOR CULTURING MDCK OR VERO CELLS OR VACCINE STRAINS OF MEASLES OR INFLUENZA VIRUSES

SERUM-FREE NUTRIENT MEDIUM FOR CULTURING MDCK OR VERO CELLS OR VACCINE STRAINS OF MEASLES OR INFLUENZA VIRUSES

机译：用于培养麻疹或流感病毒的MDCK或病毒细胞或疫苗株的无血清营养培养基

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FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, specifically to a serum-free nutrient medium, and includes a main component composition of Eagle MEM nutrient medium, and additionally contains microelements (cadmium, cobalt, zinc, nickel, selenium, molybdenum) salts in concentration of 0.00001–4.0 g/l, sodium pyruvate in concentration of 0.04–4.0 g/l, vitamins B12 and E in concentration of 0.0001–0.1 g/l, oleic acid in concentration of 0.000005–0.5 g/l, cysteine, glutathione, proline, alanine, asparagine, aspartic acid in concentration 0.001–1.5 g/l, (total 45 components), recombinant human insulin in concentration 0.000025–0.025 g/l, as well as increased concentration of leucine, phenylalanine, tryptophan, lysine to 0.035–0.072 g/l. Serum-free nutrient medium when culturing MDCK cells provides cell growth, cells have a morphology typical for the culture, a monolayer is formed on 2–3th day of growth, preserving high proliferative activity (cell culture cell proliferation index is 3.2). Production of measles virus in the Vero cell culture and influenza viruses in the MDCK cell culture in the declared serum-free medium is comparable to production of these viruses in the serum-free SFM4MegaVir medium.;EFFECT: invention enables to create a more standardized serum-free nutrient medium for culturing MDCK or Vero cells or vaccine strains of measles or influenza viruses in order to create vaccines.;1 cl, 5 dwg, 5 tbl
机译：技术领域本发明涉及生物技术，特别是涉及一种无血清营养培养基，其包含Eagle MEM营养培养基的主要成分组成，并且还含有微量元素（镉，钴，锌，镍，硒，钼）。盐的浓度为0.00001–4.0 g / l，丙酮酸钠的浓度为0.04–4.0 g / l，维生素B 12 和E的浓度为0.0001–0.1 g / l，油酸的浓度为0.000005–0.5 g / l，半胱氨酸，谷胱甘肽，脯氨酸，丙氨酸，天冬酰胺，天冬氨酸的浓度为0.001–1.5 g / l（总共45种成分），重组人胰岛素的浓度为0.000025–0.025 g / l，并​​且还会增加亮氨酸，苯丙氨酸，色氨酸，赖氨酸的浓度为0.035–0.072 g / l。培养MDCK细胞时，无血清营养培养基可促进细胞生长，细胞具有典型的培养形态，在生长的第2至3天形成单层，保留了高增殖活性（细胞培养细胞增殖指数为3.2）。在声明的无血清培养基中在Vero细胞培养物中产生麻疹病毒和在MDCK细胞培养物中产生流感病毒与在无血清的SFM4MegaVir培养基中产生这些病毒相当。效果：本发明能够创建更标准化的血清不含营养的培养基，用于培养MDCK或Vero细胞或麻疹或流感病毒的疫苗株以制备疫苗; 1 cl，5 dwg，5 tbl

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