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首页> 外国专利> METHOD FOR ASSESSING VIRULENCE IN VITRO OF TULAREMIC MICROBE STRAINS OF SUBSPECIES: FRANCISELLA TULARENSIS SUBSPECIES TULARENSIS, SUBSPECIES MEDIASIATICA, SUBSPECIES HOLARTICA

METHOD FOR ASSESSING VIRULENCE IN VITRO OF TULAREMIC MICROBE STRAINS OF SUBSPECIES: FRANCISELLA TULARENSIS SUBSPECIES TULARENSIS, SUBSPECIES MEDIASIATICA, SUBSPECIES HOLARTICA

机译：评估亚种的肺炎性微生物菌株的体外致病性的方法：费氏兰氏菌亚种TULARENSIS，MEDIASIATICA亚种，HOLARTICA亚种

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FIELD: biotechnology.;SUBSTANCE: invention relates to assessment of in vitro virulence of strains of tularemia microbe of subspecies Francisella tularensis subsp. tularensis, subsp. mediasiatica, subsp. holartica. Method involves testing the analyzed culture for the presence of bacterial LPS with the help of an indicator and a substrate, which is represented by specific antibodies of the immunochromatographic "IC-test" to LPS of the analyzed sample, which is introduced in amount of 0.1 ml into a dough with preliminary preparation of the bacterial suspension according to the industry turbidity standard and subsequent boiling at 100 °C for 30 minutes, results are recorded after 15 minutes visually by the presence of lines in the strip if two lines (one control, and the second test one) are identified, then it is concluded that the sample contains a virulent strain of one of the tuleaemia subspecies, and if one line (control) is observed, then the strain is referred to as avirulent.;EFFECT: what is presented is a method for assessing virulence in vitro of tularemic microbial strains of subspecies: francisella tularensis subspecies tularensis, subspecies mediasiatica, subspecies holartica.;1 cl, 3 tbl, 3 dwg, 2 ex
机译：发明领域：本发明涉及对弗拉氏菌亚种弗朗西斯菌亚种的杜拉氏菌微生物菌株的体外毒力的评估。图拉山亚种纵隔亚种Holartica。该方法涉及在指示剂和底物的帮助下测试被分析的培养物中细菌LPS的存在，该底物由针对被分析样品的LPS的免疫色谱法“ IC-test”的特异性抗体代表，引入量为0.1毫升，将面团按照工业浊度标准预先制备好细菌悬浮液，然后在100°C下煮沸30分钟，然后在15分钟后目视观察结果，如果有两条线，则条带中会出现线条（一个对照，并确定第二个测试），然后得出结论，该样品包含一种血球菌亚种的强毒株，如果观察到一个品系（对照），则该株被称为无毒株。提出了一种评估亚种的图莱姆微生物菌株的体外毒力的方法：弗朗西斯菌tularensis亚种tularensis，中亚亚种，holartica亚种; 1 cl，3 t bl，3 dwg，2 ex

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