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METHOD FOR DETERMINING PROLIFERATIVE ACTIVITY OF TRIPLE-NEGATIVE BREAST CANCER TUMOR CELLS
METHOD FOR DETERMINING PROLIFERATIVE ACTIVITY OF TRIPLE-NEGATIVE BREAST CANCER TUMOR CELLS
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机译：测定三阴性乳腺癌细胞增殖活性的方法
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摘要
FIELD: biotechnology.;SUBSTANCE: invention refers to medicine and biotechnology, particularly to determining the proliferative activity of tumor cells of triple-negative breast cancer. Method involves preparation of paraffin blocks from tumor tissue and histological sections, their dewaxing in hexane. Uncoloured dewaxed histological sections of tumor tissue are subjected to fluorimetric analysis and fluorescence excitation spectra are measured from 230 to 360 nm at a detection wavelength of 410 nm and fluorescence spectra at excitation wavelength of 300 nm and detection wavelength from 330 to 450 nm, determining the ratio between intensities of curves with maxima in region of 265 and 305 nm (I265/I305), and if I265/I305 = 1.0, the proliferative activity is low, the ratio I265/I305 indicates high proliferative activity.;EFFECT: invention increases efficiency of obtained results.;1 cl, 3 dwg, 1 tbl, 4 ex
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机译：技术领域本发明涉及医学和生物技术，尤其涉及确定三阴性乳腺癌的肿瘤细胞的增殖活性。方法包括从肿瘤组织和组织切片中制备石蜡块，将其在己烷中脱蜡。对肿瘤组织的无色脱蜡组织学切片进行荧光分析，在410 nm的检测波长下在230至360 nm范围内测量荧光激发光谱，在300 nm的激发波长和330至450 nm的检测波长范围内测量荧光光谱，确定在265和305 nm（I 265 / I 305 ）和（I 265 / I < Sub> 305 = 1.0，增殖活性低，I 265 / I 305 比表明增殖活性高。结果。; 1 cl，3 dwg，1 tbl，4 ex
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