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首页> 外国专利> METHOD FOR DETERMINING AMOUNT OF COLLAGEN IN TISSUE

METHOD FOR DETERMINING AMOUNT OF COLLAGEN IN TISSUE

机译：测定组织中胶原蛋白含量的方法

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摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, histology and can be used for quantitative determination of tissue collagen. Method for determining the amount of collagen in tissue involves multiple homogenisation of material obtained by freezing, lyophilic drying, milling a pre-weighed piece of tissue, followed by resuspending the material using a dispenser and centrifuging, then prepared material is frozen at -80 °C and lyophilic drying, obtained water-free material is weighed, material weight m1 is determined,material is dissolved in 900 mcl of a buffer solution with pH 7.0 and added with 100 mcl of collalisin solution, which is prepared beforehand by dissolving the contents of one ampoule of collalysin, containing collagenase in the form of lyophilizate in amount of 1.000 KE, in 2 ml of a buffer solution with pH 7.0, test tube with contents is stirred for 2 hours, three times a cycle of homogenisation, centrifugation at 13,400 rpm and resuspension of material, material is frozen at -80 °C, is lyophilically dried and weighed, material weight m2 is determined, by mass difference m1-m2 weight of collagen is determined.;EFFECT: disclosed is a method for determining the amount of collagen in tissue.;1 cl
机译：技术领域本发明涉及生物技术，组织学，并且可以用于定量测定组织胶原。确定组织中胶原蛋白含量的方法包括对材料进行多次均质处理，方法是将其冷冻，冻干，将预先称重的组织磨碎，然后使用分配器将其重悬并离心，然后将准备好的材料冷冻至-80° C并冻干，称量所得无水物料的重量，确定物料重量m 1 ，将物料溶解在900毫升的pH 7.0缓冲溶液中，并加入100毫升的Collalisin溶液，通过预先制备一个安瓿瓶的溶菌素的内容物，该溶菌素含有冻干形式的胶原酶，含量为1.000 KE，溶于2 ml pH 7.0的缓冲溶液中，将装有内容物的试管搅拌2小时，每次3次均质化周期，13,400 rpm离心力和物料重悬，物料在-80°C下冷冻，冻干并称重，通过质量差m 2 确定> 1 -m 2 胶原蛋白的重量。效果：公开了一种确定组织中胶原蛋白含量的方法。1cl

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