首页> 外国专利> METHOD OF DIFFERENTIATION OF YERSINIA PESTIS STRAINS OF MEDIEVAL BIOVAR ACCORDING TO PHYLOGENETIC AFFILIATION WITH PCR METHOD WITH ELECTROPHORETIC ACCOUNTING OF RESULTS

METHOD OF DIFFERENTIATION OF YERSINIA PESTIS STRAINS OF MEDIEVAL BIOVAR ACCORDING TO PHYLOGENETIC AFFILIATION WITH PCR METHOD WITH ELECTROPHORETIC ACCOUNTING OF RESULTS

机译:结果的亲缘鉴定,根据系统发育鉴别中生双歧杆菌的耶尔森氏菌菌株

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology and can be used in research institutions of medical profile and Rospotrebnadzor services. Invention consists in providing fast and reliable differentiation of the strains of the medieval biovar from the Y. pestis strains of other biovars and subspecies with the subsequent separation of the strains of the medieval biovar according to their phylogenetic affiliation. Technical result is achieved by the method of differentiation of the strains of the causative agent of the plague of the medieval biovar with the definition of their phylogenetic affiliation by the method of PCR with electrophoretic accounting of the results, using the corresponding primers SEQ ID NO: 1-4 on the target DNA "(-183) 2.med", "pCKF", "(-100) 2.med1" and "(-73) 2.med3", followed by differentiation according to the size of amplification in accordance with the table.;EFFECT: ensuring fast and reliable differentiation of strains of medieval biovar.;1 cl, 4 ex, 1 tbl
机译:技术领域:本发明涉及生物技术,并且可以用于医学研究和Rospotrebnadzor服务的研究机构。本发明在于提供中世纪生物变种的菌株与其他生物变种和亚种的鼠疫耶尔森氏菌菌株的快速和可靠的区分,随后根据它们的系统发生联系将中世纪生物变种的菌株分离。通过使用相应的引物SEQ ID NO:进行PCR电泳并鉴定结果的方法,通过区分中世纪生物变种鼠疫病原体的菌株并定义其系统发生亲缘关系的方法来获得技术成果。在目标DNA“(-183)2.med”,“ pCKF”,“(-100)2.med1”和“(-73)2.med3”上添加1-4,然后根据扩增大小进行区分效果:确保快速,可靠地区分中世纪生物变种的菌株。1cl,4 ex,1 tbl

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